BsaI Restriction Endonuclease
Description
The BsaI restriction enzyme is a type II restriction endonuclease derived from the recombinant protein encoded by the BspQI gene in Bacillus sphaericus expressed by E.coli. Restriction endonucleases are widely used in gene mapping, cloning and other fields. This enzyme rapidly cuts DNA for efficient gene linearization Bsa I , an IIs restriction endonuclease restriction endonuclease, is derived from a recombinant E. coli strain that carries the cloned and modified BsaI gene from Bacillus stearothermophilus. which have the same specificity as native enzymes and reduced star activity. Its recognition sequence and cleavage sites are as follows:
5'······GGTCTC(N)······ ··3'
3'······CCAGAG(NNNNN)······5'
Chemical Structure
Product features
• The restriction endonuclease has high enzymatic activity and completes digestion reaction quickly.
• Low non-specific endonuclease activity to ensure precise "scalpel" cutting.
• No BSA system, reducing heat source pollution.
Specification
| Test Items | Specifications |
| Enzyme Activity | 20 U/µL |
| Purity(SDS-PAGE) | ≥95% |
| Rnase | None detected |
| Non-Specific DNase Activity | Non detected |
| Star Activity | Non detected |
| Ligation and Recutting | After digestion, DNA fragments can be ligated. And these ligated fragments can be recut with Bsal. |
| Endotoxins(LAL-test) | ≤10 EU/mg |
| E.Coli DNA | ≤1 Copy/2U |
Quality control
Purity ≥ 95%, No DNase, RNase activity, Host DNA residue ≤ 100pg/mg, host protein residue ≤ -50ppm, endotoxin residue ≤ 10EU/mg, No protease activity, sterile, free of mycoplasma
Application
Digest the plasmid to prepare a linearized DNA fragment at the end of Poly (A/T/C/G);
Linearize plasmid template before in vitro transcription;
Restriction digest;
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of Internal control DNA in 1 hour at 37°C in a total reaction volume of 50 µL
Transportation and storage
Transportation: Shipped under -15°C
Storage: Store at -25~-15°C
Recommended re-test Life: 1 year
