CHO Residual DNA Detection Kit HYI122
HYASEN CHO Residual DNA assay Kit is used to quantitate host-cell line residual DNA from CHO cell line, which is used for production of biopharmaceutical products. This kit uses duplex real-time PCR technology to performs rapid, specific, and reliable quantitation assay at the fg level. IPC(Internal Positive Control) is included in the CHO Primer&Probe MIX to evaluate the performance of each PCR reaction. For extraction information,please refer to the HYASEN Residual Host Cell DNA Sample Preparation Kit (Cat. No.: HYI126).
Components & Storage
|
Reagent |
Quantity |
Storage |
|
CHO DNA Control |
50μL × 1 tube |
-20℃ |
|
qPCR Master MIX |
850 μL × 2 tubes |
-20℃ protect form light |
|
DNA Dilution Buffer (DDB) |
1.5 mL × 3 tubes |
-20℃ |
|
CHO Primer&Probe MIX (Incl IPC) |
500 μL × 1 tube |
-20℃ protect form light |
The kit components can be stored at appropriate conditions for up to 24 months. Please check the expiration date on the labels.
Applied instruments, including but not limited to the following
1.7500Real-Time PCR System
2.CFX96Real-Time PCR System
3.Lightcycler480Real-Time PCR System
Required materials not included in the kit
1.Nonstick, DNase-free&Low Retention Microfuge Tubes, 1.5mL
2.Nonstick, Low Retention Tips, 1000μL, 100μL, 10μL
3.96-well qPCR plates or PCR 8-strip tubes
Related equipment
1.Real-Time PCR System
2.Vortex mixer
3.Micro plate shaker
4.Pipettes, 1000μL, 100μL, 10μL
Workflow
