D-Lactate dehydrogenase(LDH)
Description
The enzyme is used for the removal of pyruvate in determinations working with NADH (triglycerides, lipase, aldolase, aspartate aminotransferases, glutamate dehydrogenase).
Usage
1.Alkaline phosphatase coupled to proteins (antibodies, streptavidin etc,) can specifically identify target molecules, and can be used in ELISA, WB and histochemical detection;
2.Alkaline phosphatase can be used to dephosphorize the 5 '-terminal of DNA or RNA to prevent self-linking;
3.The above dephosphorylated DNA or RNA can be labeled by radio-labeled phosphates (via T4 poly-nucleotide kinase)
Chemical Structure
Reaction mechanism
D-Lactate + NAD+ → Pyruvate +NADH + H+
Specification
| Test Items | Specifications |
| Description | White amorphous powder, lyophilized |
| Activity | ≥200U/mg |
| Purity(SDS-PAGE) | ≥90% |
| Solubility(10mg powder/mL) | Clear |
| NADH/NADPH oxidase | ≤0.01% |
| Malate dehydrogenase | ≤0.005% |
| Glutamate dehydrogenase | ≤0.003% |
| Pyruvate kinase | ≤0.03% |
| Glutamate Dehydrogenase | ≤0.003% |
| Aspartate aminotransferase | ≤0.001% |
| Alamine aminotransferase | ≤0.001% |
Transportation and storage
Transportation: AmbIent
Storage : Store at -20°C(Long term), 2-8°C (short term)
Recommended re-test Life: 2 year
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