DNA Library Prep Kit for ONT HYD119
DNA Library Prep Kit 2.0 is a new generation library construction kit specially developed and designed for Illumina® &MGI® sequencing platform. On the basis of the previous generation of library construction kit, this product exhibits higher efficiency in end repair, dA-tailing, and adapter ligation than the previous versions. The high-fidelity enzyme significantly improves the uniformity and fidelity of amplification. The kit is compatible with most DNA sample types, including standard genomic DNA from animals/plants/microorganisms, FFPE samples, cfDNA, and ChIP DNA.
Reagent Composition
|
Components |
24 T |
96 T |
|
Endprep Buffer |
168 μL |
672 μL |
|
Endprep Enzyme |
72 μL |
288 μL |
|
Rapid Ligase Master Mix |
840 μL |
4×840 μL |
|
Rapid Ligation Reaction Buffer（5×） |
480 μL |
2×960 μL |
|
Rapid T4 DNA Ligase |
120 μL |
480 μL |
|
Elution Buffer |
480 μL |
2×960 μL |
Storage Conditions
Store at -25°C to -15°C, with a shelf life of 1 year.
Precautions
1.The washing reagents used for purification after Native Barcode ligation and Native Adapter purification are different. Please ensure the correct reagents are used for each purification step.
2. To ensure successful library construction, it is recommended to verify the fragment length and integrity of the input DNA (validation methods include capillary electrophoresis or other equivalent methods). DNA quality (integrity, fragmentation), purity (e.g., chemical contamination, RNA residuals), and excessive or insufficient usage may affect library yield.
3. Please prepare all necessary materials in advance according to the provided guidelines to avoid issues that could prevent proper execution of the experiment, leading to sample or reagent waste.
4. When preparing libraries for multiple samples, please record the Native Barcode number corresponding to each sample.