DNase assay Kit(Fluorescence) HYJ525
The DNase detection kit is based on a fluorophore-labeled DNA probe. When the sample does not contain DNase activity, the probe is stable and does not produce a fluorescent signal; when the sample contains DNase activity, the probe is degraded, resulting in a gradual enhanced fluorescence signal; the rate of increase in fluorescence signal is positively correlated with the number and activity of enzymes. Use a fluorescence microplate reader to measure at the wavelength of ex/em=485/525nm to determine whether the sample is contaminated by DNase.
Components
|
Components |
48 T |
192 T |
|
10×reaction solution |
0.5mL |
2.0mL |
|
DNA probe |
1tube |
1tube |
|
TE buffer |
0.5mL |
2.0mL |
|
DNase I standard (2U/μL) |
10μL |
20μL |
|
Standard Dilution Buffer |
6mL |
12mL |
|
DNase＆RNase-free water |
25mL |
25mL |
|
DNase RNase away |
50mL |
50mL |
Storage
1. Transported in -25 ~ -15℃;
2. The different components of the kit are storedseparately according to the temperature：
|
Components |
Temperature |
|
10×reaction solution |
-25~-15℃ |
|
DNA probe |
-25~-15℃ |
|
TE buffer |
-25~-15℃ |
|
DNase I standard (2U/μL) |
-25~-15℃ |
|
Standard Dilution Buffer |
-25~-15℃ |
|
DNase＆RNase-free water |
-25~30℃ |
|
DNase RNase away |
2~30℃ |
3. Store the unopened kit for 12 months.
4. Store the kit for 6 months after opening. It isrecommended to aliquot the DNA probe solution according to the single use amount to avoid light and repeated freezing and thawing
Additional Materials Required
1. Fluorescence microplate reader (includingex/em=485/525nm wavelength)
2. DNase＆RNase-free pipettes and tips
3. DNase＆RNase-free EP tube
4. DNase＆RNase-free black non-transparent96-well plate