DNase I HYJ234
DNase I (deoxyribonuclease I) is an endodeoxyribonuclease that digests single-stranded or double-stranded DNA. It recognizes and cleaves phosphodiester bonds, generating monodeoxynucleotides or single-stranded or double-stranded oligodeoxynucleotides with a phosphate group at the 5' end and a hydroxyl group at the 3' end. DNase I activity is dependent on Ca2+ and can be activated by divalent metal ions such as Mn2+ and Zn2+. 5mM Ca2+ protects the enzyme from hydrolysis. In the presence of Mg2+, the enzyme can randomly recognize and cleave any site on either DNA strand. In the presence of Mn2+, it can recognize both DNA strands simultaneously and cleave at nearly identical sites, forming blunt-ended or sticky-ended DNA fragments with one to two nucleotide overhangs.
Components
|
Components |
0.1KU |
0.5KU |
5KU |
50KU |
|
DNase I, RNase-free (5U/uL) |
0.02 mL |
0.1 mL |
1 mL |
10 mL |
|
10×DNase I Buffer |
1 mL |
1 mL |
5×1 mL |
5×10 mL |
Storage
Store at -25 to -15°C. Valid for 2 years.
Storage buffer
10mM Tris-HCl, 2mM CaCl2, 50% glycerol, pH 7.6 @25℃.
Activity Definition
One unit (U) of activity refers to the amount of enzyme required to completely degrade 1 μg of pBR322 DNA at 37°C in 10 minutes.
Quality Control
1.RNase activity: 5 U of this product and 1.6 μg of MS2 RNA reacted at 37°C for 4 hours; the RNA electrophoretic band did not change.
2.Endotoxin:LAL-Test, Chinese Pharmacopoeia 2020 Edition, Part IV, Gel Limit Test, General Rules (1143), bacterial endotoxin content ≤ 10 EU/mg.