Double-stranded RNA (dsRNA) ELISA kit HYJ521
This kit is an Enzyme-Linked Immunosorbent Assay (ELISA) coupling with biotin-Streptavidin system, for quantitative measurement of dsRNA with length above 40 base pairs(bp), regardless of the sequence. The plate has been pre-coated with anti-dsRNA antibody. dsRNA present in the sample is added and binds to antibodies coated on the wells. And then biotinylated anti-dsRNA antibody is added and binds to dsRNA in the sample. After washing, HRP-Streptavidin is added and binds to the Biotinylated anti-dsRNA antibody. After incubation unbound HRP-Streptavidin is washed away. Then TMB substrate solution is added and catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of dsRNA captured in plate. The absorbance is measured at 450 nm.
Components
|
Components |
48 T |
96 T |
|
ELISA Microplate |
8×6 |
8×12 |
|
Biotinylated detection antibody (100x) |
60 μL |
120 μL |
|
HRP-streptavidin (100x) |
60 μL |
120 μL |
|
Dilution buffer |
15 mL |
30 mL |
|
TMB substrate solution |
6 mL |
12 mL |
|
Stop solution |
3 mL |
6 mL |
|
Concentrated wash buffer (20x) |
20 mL |
40 mL |
|
Standard (UTP, 1ng/μL) |
7.5 μL |
15 μL |
|
Standard (pUTP,1ng/μL) |
7.5 μL |
15 μL |
|
Standard (N1-Me-pUTP, 1ng/μL) |
7.5 μL |
15 μL |
|
Standard (5-OMe-UTP, 1ng/μL) |
7.5 μL |
15 μL |
|
STE buffer |
25 mL |
50 mL |
|
Plate sealer |
2 pieces |
4 pieces |
Storage
1. For unused kit: The whole kit could be storedat 2~8℃ in shelf life. Strong light should be avoided for storage stability.
2. For used kit: Once the microplate is opened, please cover unused wells with plate sealer andreturn to the foil pouch containing the desiccant pack, zip-seal the foil pouch and return to 2~8℃ as soon as possible after use.
3. Other reagentsshould be returned to 2~8℃ as soon as possible after use.
Additional Materials Required
1. Microplate reader with 450±10nm filter(betterif can detect at 450 and 650 nm wavelength). l
2. Microplate shaker.
3. RNase-free tips and centrifuge tubes.