E. coli Residual DNA Assay Kit HYI124
HYASEN® E.coli Residual DNA Assay Kit is used to quantitate residual E. coli host cell DNA in different stages of biopharmaceutical products, from in-process samples to final products. This kit uses duplex real-time PCR technology to perform rapid, specific, and reliable quantitation assay at the fg level. IPC (Internal Positive Control) is included in the E. coli Primer&Probe MIX to evaluate the performance of each PCR reaction. For extraction information, please refer to the HYASEN® Residual Host Cell DNA Sample Preparation Kit (Cat.No.:HYI126)
Components & Storage
|
Reagent |
Quantity |
Storage |
|
E. coli DNA Control |
50 uL x 1 tube |
-20℃ |
|
qPCR Master MIX |
850 uL x 2 tubes |
-20℃ protect form light |
|
DNA Dilution Bufer (DDB) |
1.5 mL x 3 tubes |
-20℃ |
|
E. coli Primer&Probe MlX (Incl IPC) |
500 uL x 1 tube |
-20℃ protect form light |
The kit components can be stored at appropriate conditions for up to 24 months. Please check the expiration date on the labels.
Applied instruments, including but not limited to the following
1.7500Real-Time PCR System
2.CFX96Real-Time PCR System
3.Lightcycler480Real-Time PCR System
Required materials not included in the kit
1.Nonstick, DNase-free&Low Retention Microfuge Tubes, 1.5mL
2.Nonstick, Low Retention Tips, 1000μL, 100μL, 10μL
3.96-well qPCR plates or PCR 8-strip tubes
Related equipment
1.Real-Time PCR System
2.Vortex mixer
3.Micro plate shaker
4.Pipettes, 1000μL, 100μL, 10μL
Workflow
