Kit ɗin Magnetic Universal Viral DNA/RNA na HYD521
Kit ɗin Magnetic Universal Viral DNA/RNA Kit ya dace da cire ƙwayoyin cuta masu ɗauke da ƙwayoyin cuta daga jini gaba ɗaya ko ruwan jiki mara ƙwayoyin halitta (kamar su serum, plasma, ruwan cerebrospinal, swabs na hanci/pharyngeal, ruwan wanke-wanke na alveolar, da sauransu). Wannan hanyar tana amfani da fasahar tsarkake beads na maganadisu, kuma ba a buƙatar cire phenol chloroform mai guba yayin aikin cirewa. Yana da aminci, ba shi da guba kuma yana da sauri. Ana iya amfani da samfurin da aka samu kai tsaye a cikin PCR, qPCR, jerin tsararraki na ƙarni na biyu da sauran gwaje-gwaje. Lokacin da aka yi amfani da shi tare da kayan aikin cire beads na maganadisu ta atomatik, ana iya samun babban aikin cire nucleic acid.
Bayanin samfur
| Sassan | 48T |
| Maganin Lysis da Binding | 33 ml |
| dakatarwar magnetic bead | 1.1 mL |
| Ma'ajiyar Wankewa A | 40 ml |
| Ma'ajiyar Wankewa B | 80 ml |
| Maganin fitar ruwa | 10 ml |
| Proteinase K | 1.1 mL |
Yanayin Ajiya
Ya kamata a adana Proteinase K a zafin 2 ~ 8℃, sauran sinadaran kuma a adana su a zafin ɗaki. Tsawon lokacin shiryawa shine watanni 18.
Bayanin Samfurin
1. Nau'in samfuran da suka dace: jinin gaba ɗaya, serum, plasma, ruwan cerebrospinal, swabs na hanci/pharyngeal, ruwan wanke-wanke na alveolar da sauran samfuran.
2. Ajiye samfurin da jigilar sa: Ana iya amfani da samfuran don gwaji nan take ko adana su a -70℃ ko ƙasa da haka don gwaji. Lokacin ajiya shine watanni 6. Ya kamata a guji daskarewa da narkewa akai-akai. Ana jigilar samfuran ta hanyar sarkar sanyi.
3. Bukatun daskarewa da narkewa: Daskarewa da narkewa cikin sauri, a guji daskarewa da narkewa akai-akai.
Aiki
Kafin gwajin, a duba ko ruwan yana da ruwan sama kuma ko za a iya sake dakatar da majina.
1. A juye samfurin 200 ~ 300 μL zuwa bututun centrifuge mai 1.5 mL, a ƙara 600 μL na Lysis da Binding Solution, 20 μL na Proteinase K, da 20 μL na magnet beads a jere, sannan a yi vortex a babban gudu na daƙiƙa 30. A saka a 50℃ na tsawon mintuna 5 ~ 7. Idan thermostat ɗin ba shi da aikin juyawa, a yi vortex sau 3 a lokacin shiryawa, kowane lokaci na daƙiƙa 15.
2. A canza zuwa wurin maganadisu mai girman 1.5 mL don raba maganadisu har sai maganin ya bayyana kuma ya bayyana, sannan a jefar da maganin.
3. A ƙara 700 μL na abin wankewa A, a yi vortex na tsawon daƙiƙa 10 don a karya ƙwallayen maganadisu, sannan a canja zuwa wurin da za a iya raba maganadisu har sai maganin ya bayyana, sannan a zubar da maganin.
4. A ƙara 700 μL na abin wankewa na B, a zuba vortex na tsawon daƙiƙa 10 don ya wargaza ƙwallayen maganadisu, a mayar da su zuwa wurin da za a iya raba magnetic har sai maganin ya bayyana, sannan a zubar da maganin.
5. Sai a sake ƙara 700 μL na abin wankewa na B, a juya na tsawon daƙiƙa 10 don ya wargaza ƙusoshin maganadisu, a mayar da su zuwa wurin da za a iya raba maganadisu har sai maganin ya bayyana, sannan a zubar da maganin.
6. A yi amfani da na'urar centrifuge na ɗan lokaci don tattara ɗigon ruwa a bangon bututun, a mayar da shi zuwa wurin maganadisu don a yi masa haske, sannan a sha ruwan da ya rage. A busar da shi a iska na tsawon minti 3-5.
Lura: Ragowar ethanol zai hana amsawar enzyme daga baya, don haka a tabbatar cewa ethanol ɗin ya ƙafe gaba ɗaya lokacin bushewa. Kada a bushe na dogon lokaci don guje wa shafar tasirin fitar da ruwa daga baya.
7. Ƙara 40 ~ 100 μL na ruwan Elution, a zuba vortex a babban gudu na tsawon minti 2 ~ 3 don ya karya magnetic beads ɗin. A zuba a zafin 60℃ na tsawon minti 5, sannan a zuba vortex a babban gudu na tsawon daƙiƙa 60.
8. Nan take a zuba na'urar centrifuge domin tattara ruwan daga murfin bututun sannan a mayar da shi zuwa ga akwatin maganadisu har sai an cika mannewar magnesit ɗin gaba ɗaya. Sannan a hankali a mayar da ruwan zuwa wani sabon bututun centrifuge don samun maganin nucleic acid.
9. Ana iya adana maganin nucleic acid a -20°C don adanawa na ɗan gajeren lokaci da kuma -80°C don adanawa na dogon lokaci.
Bayanan kula
1. Ma'ajiyar sinadarai daban-daban da ke cikin wannan kayan aikin tana ɗauke da gishirin guanidine. Don lafiyarka da lafiyarka, da fatan za a saka rigar dakin gwaje-gwaje da safar hannu da za a iya zubarwa. Kuma a riƙe ta bisa ga ƙa'idodin tsaro. Kada a bari ma'ajiyar ta taɓa fata, idanu da kuma maƙogwaro. Idan hakan ta faru, da fatan za a wanke nan da nan da ruwa mai yawa sannan a nemi taimakon likita.
2. Idan ruwan ya yi ambaliya, sai a wanke shi da ruwa mai zafin ℃ 30 har sai ruwan ya narke gaba ɗaya kafin a yi amfani da shi.
3. Idan an daskare murfin murfin maganadisu, kada a yi amfani da shi.
4. Ma'adanai daban-daban da ke cikin wannan kayan suna ɗauke da gishirin guanidine. Kada a yi amfani da magungunan kashe ƙwayoyin cuta kamar sodium hypochlorite don magance su, in ba haka ba za a fitar da iskar gas mai guba kuma dole ne a ɗauke su a matsayin sharar magani.
5. Akwai yiwuwar samun ragowar ƙusoshin maganadisu yayin fitar da ruwa. Yi ƙoƙarin guje wa shaƙar ƙusoshin maganadisu yayin da kake shaƙa samfuran numfashi.