HEK293 Residual DNA Detection Kit HYI121
HYASEN® HEK293 Residual DNA Detection Kit is used to quantitate residual HEK293 host cell DNA in different stages of biopharmaceutical products, from in-process samples to final products. This kit uses duplex real-time PCR technology to performs rapid, specific, and reliable quantitation assay at the fg level. IPC(Internal Positive Control) is included in the HEK293 Primer&Probe MIX to evaluate the performance of each PCR reaction. For extraction information, please refer to the HYASEN® Residual Host Cell DNA Sample Preparation Kit (Cat. No.: HYI126).
Components & Storage
|
Reagent |
Quantity |
Storage |
|
HEK293 DNA Control |
50 uL x 1 tube |
-20℃ |
|
qPCR Master MIX |
850 uL x 2 tubes |
-20℃ protect form light |
|
DNA Dilution Bufer (DDB) |
1.5 mL x 3 tubes |
-20℃ |
|
HEK293 Primer&Probe MlX (Incl IPC) |
500 uL x 1 tube |
-20℃ protect form light |
The kit components can be stored at appropriate conditions for up to 24 months. Please check the expiration date on the labels.
Applied instruments, including but not limited to the following
1.7500Real-Time PCR System
2.CFX96Real-Time PCR System
3.Lightcycler480Real-Time PCR System
Required materials not included in the kit
1.Nonstick, DNase-free&Low Retention Microfuge Tubes, 1.5mL
2.Nonstick, Low Retention Tips, 1000μL, 100μL, 10μL
3.96-well qPCR plates or PCR 8-strip tubes
Related equipment
1.Real-Time PCR System
2.Vortex mixer
3.Micro plate shaker
4.Pipettes, 1000μL, 100μL, 10μL
Workflow
