Homocysteine(HCY) is used to detect homocysteine in human blood. Homocysteine (Hcy) is a sulfur-containing amino acid produced by the metabolism of methionine. 80% of Hcy is bound to proteins through disulfide bonds in the blood, and only a small part of free homocysteine participates in the circulation. Hcy levels are closely related to cardiovascular diseases. is an important risk factor for cardiovascular disease. The increased Hcy in the blood stimulates the blood vessel wall to cause damage to the arterial vessel, leading to inflammation and plaque formation on the vessel wall, which eventually leads to blockage of blood flow in the heart. In patients with hyperhomocystinuria, severe genetic defects affect Hcy metabolism, resulting in hyperhomocysteinemia. Mild genetic defects or nutritional deficiencies of B vitamins will be accompanied by moderate or mild elevation of Hcy, which will also increase the risk of heart disease. Elevated Hcy can also cause birth defects such as neural tube defects and congenital malformations.
Oxidized Hcy is converted into free Hcy, and free Hcy reacts with serine under the catalysis of CBS to generate L-cystathionine. L-cystathionine generates Hcy, pyruvate and NH3 under the catalysis of CBL. The pyruvate generated by this cycle reaction can be detected by lactate dehydrogenase LDH and NADH, and the conversion rate of NADH to NAD is directly proportional to the Hcy content in the sample.
Transportation and storage
Storage and validity period: Unopened reagents should be stored at 2-8°C in the dark, and the validity period is 12 months; after opening, the reagents should be stored in the dark at 2-8°C, and the validity period is 1 month under the condition of no pollution; the reagents should not be frozen.
Sample requirements: The sample is fresh serum or plasma (heparin anticoagulation, 0.1mg heparin can anticoagulate 1.0ml blood). Please centrifuge the plasma immediately after blood collection, or refrigerate and centrifuge within 1 hour.