Hyaseq DNA Library Prep Kit V2 HYD111
Hyaseq DNA Library Prep Kit V2 is a new generation library preparation kit specially developed and designed for Illumina&MGI sequencing platform. On the basis of the previous generation of library preparation kit, this product exhibits higher efficiency in end repair, dA-tailing, and adapter ligation than the previous versions. The high-fidelity enzyme significantly improves the uniformity and fidelity of amplification. The kit is compatible with most DNA sample types, including standard genomic DNA from animals/plants/microorganisms, FFPE samples, cfDNA, and ChIP DNA.
Components
|
Components |
24T |
96T |
|
DNA End Repair Buffer V2 |
168 μL |
672 μL |
|
DNA End Repair Enzyme Mix V2 |
72 μL |
288 μL |
|
Ligation Enhancer V2 |
720 μL |
3×960 μL |
|
Rapid T4 DNA Ligase V2 |
240 μL |
2×480 μL |
|
2 × NGS Library Amplification Mix |
600 μL |
3×800 μL |
Note: If the recommended short adapters are used, the Primer Mix is not required. However, if complete adapters are selected, the Primer Mix is essential but not included in this kit; it must be prepared separately. While the components of this kit are compatible with both Illumina and MGI platforms, a platform-specific Primer Mix must be purchased separately: Cat.No.: HYD313 Primer Mix for Illumina or Cat.No.: HYD317 Primer Mix for MGI.
Storage
Store at -25 ~ -15°C, valid for 1 year.