Hyaseq Ultra DNA Library Prep Kit V4 HYD113
Hyaseq Ultra DNA Library Prep Kit V4 is a next-generation enzymatic library preparation kit designed for use with Illumina and MGI high-throughput sequencing platforms. Compared to traditional library preparation methods, this kit utilizes high-quality fragmentation enzymes, eliminating the need for cumbersome sonication processes. The fragmentation and end-repair modules are combined into a single step, significantly reducing both the time and cost of library preparation. This kit is suitable for a wide range of sample types, including plant and animal genomes, microbial genomes, and more, with a sample input range of 1 ng to 1 μg. The enzymatic fragmentation ensures uniform fragment sizes across different species, with minimal inter-species variation. Additionally, this kit can be paired with Illumina or MGI adapters and primers for sequencing on Illumina or MGI high-throughput platforms.
Components
|
Components |
24T |
96T |
|
Fragment/End Repair Buffer V4 |
240 μL |
960 μL |
|
Fragment/End Repair Enzyme Mix V4 |
240 μL |
960 μL |
|
Ligation Enhancer V4 |
720 μL |
3×960 μL |
|
Rapid DNA Ligase V4 |
240 μL |
2×480 μL |
|
2× HiFi Amplification Mix |
600 μL |
3×800 μL |
Storage
Store at -25 ~ -15℃ for 1 year.

