LAMP Fluorescent Master Mix (Lyophilized beads） HYB315
LAMP Fluorenscent Master Mix (Lyophilized beads）contains reaction buffer, Bst DNA polymerase, and freeze-dried protective agent. The reaction buffer contains Mg2+, dNTP and other necessary components for amplification. The product is a freeze-dried pellet formulation, and only primers and templates need to be added when using it.
Components
|
Components |
96 T |
960 T |
9600 T |
|
LAMP Fluorenscent Master Mix (Lyophilized beads） |
8T/strip×12 |
8T/strip×120 |
8T/strip×1200 |
Storage
Store at 2-8℃，valid for 12 months.
Instructions
1. Take out the corresponding number of lyophilized beads according to the number of tests.
2. Preparation of reaction system
|
Component |
Volume |
|
LAMP Fluorenscent Master Mix (Lyophilized beads） |
One piece (two beads) |
|
10×Primer Mix* |
5 μL |
|
Template DNA** |
Up to 50 μL |
*10× Primer Mix concentration: 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B.
**It is recommended to dissolve the nucleic acid template in DEPC water.
3. Amplification reaction
Set up the following program on a fluorescent PCR instrument (such as ABI7500, etc.).
|
Temperature |
Time |
Cycles |
|
65℃ |
60s *Collect fluorescent signal |
30 |
Notes
1. The reaction temperature can be optimized between 62℃-68℃ according to the primer situation;
2. The experiment should be operated in a standardized manner, including the preparation of the reaction system, sample processing and sample addition;
3. To avoid contamination, it is recommended to prepare the reaction system in a clean bench and add the template in a fume hood in other rooms to avoid false positive interference.