LAMP HNB Colorimetric Master Mix (Lyophilized beads） HYB313
This product contains reaction buffer, Bst DNA polymerase, lyophilized protectant and chromogenic dye components. The reaction buffer contains Mg2+, dNTP and other components necessary for amplification. The product is lyophilized beads, and only templates need to be added for use. This Kit provides a fast, clearly visual detection of amplification, which negative reaction is indicated in bluish violet and positive reaction in indicated by a change to sky blue.
Components
|
Components |
96T |
960T |
9600T |
|
LAMP HNB Colorimetric Master Mix (Lyophilized beads） |
8 T/strip×12 |
8 T/strip×120 |
8 T/strip×1200 |
Storage
Store at 2-8℃，valid for 12 months.
Instructions
1. Take out the corresponding number of lyophilized beads according to the number of tests.
2. Prepare the reaction system
|
Components |
Volume |
|
LAMP HNB Colorimetric Master Mix (Lyophilized beads） |
One piece |
|
10×Primer Mix* |
2.5 μL |
|
Template DNA** |
Up to 25uL |
*10× Primer Mix: 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B.
**It is recommended to dissolve the nucleic acid template in DEPC water.
3. Incubate at 65°C for 30-45 minutes. The reaction time can be appropriately extended according to the color change.
4. According to the naked eye, sky blue was positive and bluish violet was negative.
Notes
1. The reaction temperature can be optimized between 62℃-68℃ according to the primer situation;
2. The reagents after aliquoting should be avoided from being exposed to the air for a long time;
3. The experiment should be operated in a standardized manner, including the preparation of the reaction system, sample processing and sample addition;
4. To avoid contamination, it is recommended to prepare the reaction system in a clean bench and add the template in the fume hood in other rooms to avoid false positive interference.