Magnetic Fast Blood DNA Kit-HYC227
The workflow eliminates toxic phenol-chloroform extraction and time-consuming ethanol precipitation, and the whole extraction procedure takes only 40 minutes. DNA purified by this method covers genomic DNA, mitochondrial DNA, viral DNA (e.g., Hepatitis B virus DNA), as well as DNA from other parasitic microorganisms. The recovered DNA can be directly applied to PCR, viral DNA detection and other downstream assays.
Components
|
Components |
48 T |
96 T |
|
Proteinase K Solution |
1.2 mL |
2.5 mL |
|
Magnetic Microspheres |
1.7 mL |
3.5 mL |
|
Buffer MLA |
30 mL |
60 mL |
|
Buffer MWX1 |
30 mL |
70 mL |
|
Buffer DW1 |
30 mL |
70 mL |
|
Buffer EW |
60 mL |
120 mL |
|
Elution Buffer |
10 mL |
20 mL |
Storage
Proteinase K Solution and Magnetic Microspheres shall be stored at 2–8 °C; all other reagents are stored at room temperature. The shelf life is 18 months.
Instructions
Procedure
- Add 20 μL Proteinase K Solution and 30 μL Magnetic Microspheres into a 1.5 mL centrifuge tube.
- Load sample into the tube containing magnetic beads: 200–300 μL whole blood, 200 μL concentrated blood, 200 μL buffy coat, 300 μL saliva, 300 μL swab eluate, 200–300 μL homogenate, 300 μL digested lysate, or 200 μL cell suspension.
- Add 500 μL Buffer MLA, vortex for 10–15 sec, and incubate at ambient temperature for 10 min with several inversions during incubation. Place the tube on a magnetic rack for 1 min to capture beads, then pour out or aspirate all liquid.
- Add 500 μL Buffer MWX1 and vortex for 15 sec. Set on the magnetic rack for 1 min to capture beads, then discard all liquid.
- Add 500 μL Buffer DW1 and vortex for 15 sec. Set on the magnetic rack for 1 min to capture beads, then discard all liquid.
- Add 500 μL Buffer EW and vortex for 15 sec. Set on the magnetic rack for 1 min to capture beads, then discard all liquid.
- Add another 500 μL Buffer EW and vortex for 15 sec. Set on the magnetic rack for 1 min to capture beads, then discard all liquid.
- Perform brief centrifugation to collect liquid droplets on tube walls, place the tube on the magnetic rack, and aspirate all residual liquid completely. Air-dry magnetic beads for 10 min.
- Add 100 μL Elution Buffer and vortex thoroughly to resuspend beads. Incubate with high-speed shaking (>1000 rpm) at 55 °C for 10 min.
- Place the tube on the magnetic rack for 2 min to separate beads, then transfer purified DNA supernatant to a new centrifuge tube.