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Magnetic Micropheres B HYC125

Magnetic bead-based nucleic acid purification technology uses nano-micron superparamagnetic materials as the matrix, generally black ferric oxide or yellow-brown ferric oxide as the magnetic material. The surface of the microbeads is coated with appropriate functional groups, which can react with nucleic acids for adsorption.

Cat No.: HYC125

Specification: 10mL/100mL

    Product Description

    Magnetic bead-based nucleic acid purification technology uses nano-micron superparamagnetic materials as the matrix, generally black ferric oxide or yellow-brown ferric oxide as the magnetic material. The surface of the microbeads is coated with appropriate functional groups, which can react with nucleic acids for adsorption. Magnetic beads commonly used for nucleic acids include carboxyl-containing magnetic beads, hydroxyl-containing magnetic beads, and silanol-containing magnetic beads. Silicon dioxide containing silanol in the matrix is the most common, and its principle of adsorbing nucleic acids is consistent with the classic glass milk purification technology or glass fiber filter membrane purification method. Magnetic Microspheres B is a monodisperse spherical carboxyl magnetic bead prepared by the sandwich method. The core is a 0.6um polystyrene microbead with uniform particle size, the middle layer is a nano-scale magnetic response layer of ferric oxide, and the surface is a carboxyl-rich phenylethylene polymer. This product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.

    Components

    Components

    Size-1

    Size-2

    Magnetic Microspheres B (10mg/ml)

    10mL

    100mL

    Storage

    Store at 2-8℃, valid for 24 months.

    Purification Principle

    Alcohol-mediated binding: In solutions containing alcohol (~40%) or polyethylene glycol, DNA/RNA will be deposited and adsorbed to the surface of Magnetic Microspheres B, while impurities such as proteins will not be adsorbed. After the biological sample is treated with digestion solution or lysis solution, DNA/RNA is released from the cell, organelles, and protein complexes (ribosomes, nucleosomes) into the reagent. After adding Magnetic Microspheres B and binding solution, DNA/RNA is adsorbed to the surface of Magnetic Microspheres B to form a DNA-magnetic bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and impurities such as proteins are removed with the waste liquid. After two or three steps of further washing, the DNA-magnetic bead complex is resuspended in sterile water or TE Buffer, and the DNA falls off the surface of the magnetic beads, thereby achieving the purpose of purification.

    Specification

    Magnetic bead concentration

    10mg/ml

    Appearance

    Suspension of yellow-brown particles

    Surface functional group

    –COOH

    Decentrality

    Monodesperse,Sphere

    Particle size

    0.8~1um

    Magnetic response speed

    120 s

    Settling velocity

    >2 h

    High salt mediated

    No adsorption

    Alcohol mediated

    2M Guanidine HCl/isopropanol (40%), DNA/RNA recovery up to 90%

    PEG8000 mediated binding

    DNA/RNA recovery rate is as high as 90%.

    DNase/RNase

    undetected

    DNA redisue

    undetected

    Recommended application

    Plasmid extraction, gel DNA recovery, genomic DNA extraction, RNA extraction

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