Magnetic Microspheres HYC124
Magnetic Micropheres nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.
Components
|
Components |
Size-1 |
Size-2 |
Size-3 |
|
Magnetic Microspheres (100mg/ml) |
5mL |
100mL |
380mL |
Storage
Store at 2-8℃, valid for 24 months.
Purification Principle
1. High salt-mediated binding:In a solution containing 2-4M guanidine isothiocyanate, Magnetic Microspheres can selectively recover DNA molecules, and impurities such as proteins and polysaccharides are not adsorbed.
2. Alcohol-mediated binding: In a solution containing guanidine salts and alcohols (~25%), Magnetic Microspherescan selectively recover DNA/RNA molecules, and impurities such as proteins are not adsorbed. After the biological sample is treated with digestion solution or lysis solution, DNA/RNA is released from the cell, organelles, and protein complexes (ribosomes, nucleosomes) into the reagent. After adding Magnetic Microspheres and binding solution, DNA/RNA is adsorbed to the surface of Magnetic Microspheres to form a DNA-magnetic bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and impurities such as proteins are removed with the waste liquid. After two or three steps of further washing, the DNA-magnetic bead complex is resuspended in sterile water or TE Buffer, and the DNA falls off the surface of the magnetic beads, thereby achieving the purpose of purification.
Specification
|
Magnetic bead concentration |
100mg/ml |
|
Appearance |
Suspension of black particles |
|
Surface functional group |
Si-OH |
|
Decentrality |
Polydisperse amorphous |
|
Particle size |
1-3 um |
|
Magnetic response speed |
15-30 s |
|
Settling velocity |
>3 min |
|
High salt mediated |
>2M guanidine thiocyanate, DNA recovery rate up to 80% |
|
Alcohol mediated |
2M Guanidine HCl/isopropanol (30%), DNA/RNA recovery up to 85% |
|
PEG8000 mediated binding |
DNA/RNA recovery rate is as high as 85%. |
|
DNase/RNase |
undetected |
|
DNA redisue |
<1ppm |
|
Recommended application |
Plasmid extraction, gel DNA recovery, genomic DNA extraction, RNA extraction |