Magnetic Universal Viral DNA/RNA Kit HYD521
Magnetic Universal Viral DNA/RNA Kit is suitable for extracting viral nucleic acid from whole blood or cell-free body fluids (such as serum, plasma, cerebrospinal fluid, nasal/pharyngeal swabs, alveolar lavage fluid, etc.). This method uses magnetic bead purification technology, and no toxic phenol chloroform extraction is required during the extraction process. It is safe, non-toxic and fast. The obtained product can be directly used in PCR, qPCR, second-generation sequencing and other experiments. When used with magnetic bead automated extraction instruments, high-throughput extraction of nucleic acids can be achieved.
Product information
|
Components |
48T |
|
Lysis and Binding Solution |
33 mL |
|
Magnetic bead suspension |
1.1 mL |
|
Washing buffer A |
40 mL |
|
Washing buffer B |
80 mL |
|
Elution solution |
10 mL |
|
Proteinase K |
1.1 mL |
Storage Conditions
Proteinase K should be stored at 2~8℃ and the other ingredients should be stored at room temperature. The shelf life is 18 months.
Sample Description
1. Applicable specimen types: whole blood, serum, plasma, cerebrospinal fluid, nasal/pharyngeal swabs, alveolar lavage fluid and other samples.
2. Specimen storage and transportation: Specimens can be used for testing immediately or stored at -70℃ or lower for testing. The storage period is 6 months. Repeated freezing and thawing should be avoided. Specimens are transported by cold chain.
3. Freeze-thaw requirements: Quick freezing and thawing, avoid repeated freezing and thawing.
Operation
Before the experiment, check whether the solution has precipitation and whether the magnetic beads can be resuspended.
1. Transfer 200~300 μL of sample to a 1.5 mL centrifuge tube, add 600 μL of Lysis and Binding Solution, 20 μL of Proteinase K, and 20 μL of magnetic bead suspension in sequence, and vortex at high speed for 30 seconds. Incubate at 50℃ for 5~7 minutes. If the thermostat does not have an oscillation function, vortex 3 times during the incubation period, each time for 15 seconds.
2. Transfer to a 1.5 mL magnetic stand for magnetic separation until the solution is clear and transparent, and discard the solution.
3. Add 700 μL of wash buffer A, vortex for 10 seconds to break up the magnetic beads, and then transfer to a magnetic stand for magnetic separation until the solution is clear, and discard the solution.
4. Add 700 μL of wash buffer B, vortex for 10 s to break up the magnetic beads, transfer to a magnetic stand for magnetic separation until the solution is clear, and discard the solution.
5. Add 700 μL of wash buffer B again, vortex for 10 s to break up the magnetic beads, transfer to a magnetic stand for magnetic separation until the solution is clear, and discard the solution.
6. Centrifuge briefly to collect droplets on the tube wall, transfer to a magnetic stand for clarification, and absorb the residual liquid. Air dry for 3-5 min.
Note: Ethanol residue will inhibit subsequent enzyme reactions, so make sure that the ethanol evaporates completely when drying. Do not dry for too long to avoid affecting the subsequent elution effect.
7. Add 40~100 μL of Elution solution, vortex at high speed for 2~3 min to break up the magnetic beads. Incubate at 60℃ for 5 min, then vortex at high speed for 60 s.
8. Instant centrifuge to collect the liquid from the tube cap and transfer it to the magnetic rack until the magnetic beads are completely adsorbed. Then carefully transfer the liquid to a new centrifuge tube to obtain the nucleic acid solution.
9.The nucleic acid solution can be stored at -20°C for short-term storage and -80°C for long-term storage.
Notes
1. The various buffers in this kit contain guanidine salts. For your safety and health, please wear a lab coat and disposable gloves. And handle according to safety standard precautions. Do not let the buffer come into contact with the skin, eyes and mucous membranes. If it does happen, please wash immediately with plenty of water and seek medical attention.
2. If the solution precipitates, it needs to be bathed in 30℃ water until the precipitate is completely dissolved before use.
3. If the magnetic bead suspension is frozen, do not use it.
4. The various buffers in this kit contain guanidine salts. Do not use oxidizing disinfectants such as sodium hypochlorite to treat them, otherwise toxic gases will be released and they must be treated as medical waste.
5. There may be residual magnetic beads during elution. Try to avoid inhaling magnetic beads when aspirating samples.