N-Glycan Kit (96-well plate) HYI142
This document provides information regarding the general care and use of N-Glycan Kit for the fast enzymatic release and rapid labeling of N-glycans. This protocol is validated using monoclonal antibodies and has also been tested to perform for a wide range of other N-linked glycoproteins. We recommend the user to confirm enzymatic release for their particular sample.
Components
|
Module |
Reagent |
24 T |
96 T |
|
Deglycosylation Module |
IgG |
1.2 mg |
5 mg |
|
PNGase F |
45µL |
150 µL |
|
|
PNGase F buffer |
0.5mL |
1 mL |
|
|
Surfactant |
1.2mg*6 |
3.6 mg*8 |
|
|
Labeling Module |
MS reagent powder |
8.2mg*3 |
24.6mg*4 |
|
Anhydrous DMF |
1mL |
1mL |
|
|
Clean-up Module |
Elution Plate (96 well) |
1 piece |
1 piece |
|
Elution Buffer |
5mL*1 |
5mL*4 |
|
|
Sample Diluent |
5mL*1 |
5mL*4 |
|
|
Collection Module |
8-tube Strips (200μL） |
100 pieces |
100 pieces |
|
8-cap Strips |
100 pieces |
100 pieces |
|
|
Collection plate(96 well) |
1 pieces |
1 pieces |
|
|
Waste tray |
1 pieces |
1 pieces |
Transportation and storage
|
Reagent |
Storage |
|
IgG |
2-8℃, 12 months |
|
PNGase F |
-25~-15℃℃ , 12 months , avoid freeze/thaw cycles |
|
PNGase F buffer |
2-8℃, 12 months |
|
Surfactant |
Room Temperature, 12 months |
|
MS reagent Powder |
2-8℃, 12 months,keep in dark |
|
Anhydrous DMF |
Room temperature, 12 months |
|
Elution Buffer |
Room temperature, 12 months |
|
Sample diluent |
Room temperature, 12 months |
|
96 well elution plate |
Room temperature，once opened, the unused well need to be sealed with the plastic paraffin film and keep desiccant at room temperature |
|
200μL strips of 8tubes, strips of 8 caps, 96 well collection plate, waste tray |
Room temperature，96 well collection plate be sealed and keep desiccant at room temperature |