N-Glycan Kit (InstantPC) HYI144
This document provides information regarding the general care and use of N-Glycan Kit for the fast enzymatic release and rapid labeling of N-glycans. This protocol is validated using monoclonal antibodies and has also been tested to perform for a wide range of other N-linked glycoproteins. We recommend the user to confirm enzymatic release for their particular sample.
Components and storage
|
Module |
Reagent |
Description |
Storage |
|
Deglycosylation Module |
IgG |
For users to trouble shooting and process validation |
2-8℃, 12 months |
|
PNGase F |
For rapid deglycosylation |
-25～-15℃，12 months，avoid freeze/thaw cycles |
|
|
PNGase F buffer |
For rapid deglycosylation |
2-8℃, 12 months |
|
|
Surfactant (SF) |
enzyme-friendly surfactant used to denature the glycoproteins |
Room Temperature, 12 months |
|
|
Labeling Module |
HIP powder |
For samples Labeling |
2-8℃, 12 months, keep in dark |
|
Anhydrous DMF |
As the resolution reagent of label |
Room temperature, 12 months |
|
|
Clean-up Module |
SPE column |
For removing potential interferences |
Room temperature, 12 months |
|
Elution Buffer |
For Optimum glycan recovery |
Room temperature, 12 months |
|
|
Sample Diluent |
For diluting SPE eluste |
Room temperature, once opened, the unused column need to be sealed. |
Notes
1.For your safety and health, please wear lab coat and gloves.
2.Use clean tips each time.
3.Please follow the manual to store and use the reagents.
4.Mass added to glycan with a free reducing end: Mass of Glycan (free reducing end) + C14N3O2H19(261.14773 Da)= Mass of HIP-Labeled Glycan
Figure：IgG data get from ACQUITY® RDa HILIC-FLR-MS