Hyasen Plant DNA Kits: Your Solution for Tough Plant Samples

Plants produce a vast array of metabolites—including polyphenols, polysaccharides, and various secondary metabolites—that can severely interfere with DNA extraction. For this reason, isolating high-quality genomic DNA from plant samples is critical to the success of downstream applications.

• Polyphenol interference: During cell lysis, polyphenols oxidize, leading to DNA degradation or modification.
• Polysaccharide interference: Polysaccharides share structural similarities with DNA, making them hard to remove. They often co-purify with DNA and inhibit PCR amplification efficiency.

Hyasen Plant DNA Kits combine silica column purification with the classic CTAB/chloroform method to rapidly isolate high-purity genomic DNA from a wide range of samples—including field crops, woody plants, and hard-to-extract species. The purified DNA is ready for direct use in:RAPD, AFLP, SSC, PCR, Southern blotting, enzymatic reactions, and transgenic detection assays.

Performance Highlights

Isolating DNA from Polyphenol-Rich Woody Plants

Take 100 mg of fresh leaves from a woody plant (high in polyphenols), grind into a fine powder with liquid nitrogen, and extract using the Spin Plant DNA Mini Kit. After extraction, load 5% of the purified DNA onto a 0.8% agarose gel to verify DNA integrity via electrophoresis.

Isolating DNA from Polysaccharide-Rich Plants

Take 100 mg of leaves or tubers from a polysaccharide-rich plant, grind into a fine powder with liquid nitrogen, and extract using the Spin Plant DNA Mini Kit. OD value analysis confirms:

High purity: OD260/280 ratio between 1.7–2.0

High yield: Consistent DNA recovery across sample types