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One-Tube Rapid Detection! 2x One-Step RT-qPCR Premix-UNG Fully Premixed High-Stability Reagent

2025-12-12

The RT-qPCR workflow that reduces prep time, human error, and variability — all while keeping high sensitivity!

Hyasen Biotech proudly introduces the 2x One-Step RT-qPCR Premix-UNG. This formulation allows you to pre-mix primers and probes in a single tube before use. During detection, you simply add the RNA template and start the run — speeding up complex experimental workflows, reduces hands-on steps, and makes daily laboratory routines much easier.

Product Highlights

  • Fully Premixed, Ultra-Long Stability

This reagent allows complete pre-mixing of primers and probes. After preparation, the mix remains stable for 7 days at 37°C and 109 days at 4°C with consistent performance. You do not need to prepare the premix repeatedly, which helps reduce preparation errors and improves overall experiment success rates.

  • High Precision & Sensitivity

It uses an antibody-based hot-start Taq enzyme with an optimized buffer formulation. This combination provides high detection sensitivity, allowing reliable identification of low-concentration targets and helping to avoid false negatives.

  • UNG Enzyme for Anti-Contamination

The added UNG enzyme removes leftover PCR products, preventing carryover contamination and helping ensure that your results remain accurate and reliable.

Partial Performance Demonstration

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After pre-mixing the primers and probes with HYA438, we performed stability tests using reagents stored at −20 °C as the control. The results show that the premixed reagent remains stable for 7 days at 37 °C and for 109 days at 4 °C, with no significant performance loss. This confirms that the product is suitable for long-term storage and for preparing large premix batches.

Product Information

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