NGS Tagment Index Kit for Illumina (384 Index) HYD343
NGS Tagment Index Kit for Illumina is a special kit for Fast Tagment DNA Library Prep Kit for Illumina.This series includes 16 i5 Index Primers (T5XX) and 24 i7 Index Primers (T7XX), which can be combined with each other to construct a total of 384 different double-end Index libraries. The kit is divided into 3 specifications, which can construct 24 (4×6), 96 (8×12) and 384 (16×24) double-end Index libraries respectively. All reagents provided in the kit have undergone strict quality control and functional verification, which guarantees the stability and repeatability of library construction to the greatest extent.
Reagent Composition
|
Cat.No. |
Specification |
Components |
Size |
|
HYD341 |
24 (4×6) double-end Index |
T501-T504 |
12 μL each |
|
T701-T706 |
8 μL each |
||
|
HYD342 |
96 (8×12) double-end Index |
T501-T508 |
24 μL each |
|
T701-T712 |
16 μL each |
||
|
HYD343 |
384 (16×24) double-end Index |
T501-T516 |
48 μL each |
|
T701-T724 |
32 μL each |
Storage Conditions
All the components are shipped with dry ice and can be stored at -15℃~ -25℃ for 18 months.
Index Sequence
|
Components |
Sample Sheet Input/Sequencing Index Sequence |
||
|
NovaSeq 6000 v1.0 reagents, MiSeq, HiSeq 2000/2500 |
NovaSeq 6000 v1.5 reagents，MiniSeq, NextSeq,HiSeq 3000/4000 |
||
|
T5XX Index 2（i5） |
T501 |
CTCTCTAT |
ATAGAGAG |
|
T502 |
TATCCTCT |
AGAGGATA |
|
|
T503 |
GTAAGGAG |
CTCCTTAC |
|
|
T504 |
ACTGCATA |
TATGCAGT |
|
|
T505 |
AAGGAGTA |
TACTCCTT |
|
|
T506 |
CTAAGCCT |
AGGCTTAG |
|
|
T507 |
CGTCTAAT |
ATTAGACG |
|
|
T508 |
TCTCTCCG |
CGGAGAGA |
|
|
T509 |
TCGACTAG |
CTAGTCGA |
|
|
T510 |
TTCTAGCT |
AGCTAGAA |
|
|
T511 |
CCTAGAGT |
ACTCTAGG |
|
|
T512 |
GCGTAAGA |
TCTTACGC |
|
|
T513 |
CTATTAAG |
CTTAATAG |
|
|
T514 |
AAGGCTAT |
ATAGCCTT |
|
|
T515 |
GAGCCTTA |
TAAGGCTC |
|
|
T516 |
TTATGCGA |
TCGCATAA |
|
|
T7XX Index 1（i7） |
T701 |
TAAGGCGA |
TAAGGCGA |
|
T702 |
CGTACTAG |
CGTACTAG |
|
|
T703 |
AGGCAGAA |
AGGCAGAA |
|
|
T704 |
TCCTGAGC |
TCCTGAGC |
|
|
T705 |
GGACTCCT |
GGACTCCT |
|
|
T706 |
TAGGCATG |
TAGGCATG |
|
|
T707 |
CTCTCTAC |
CTCTCTAC |
|
|
T708 |
CGAGGCTG |
CGAGGCTG |
|
|
T709 |
AAGAGGCA |
AAGAGGCA |
|
|
T710 |
GTAGAGGA |
GTAGAGGA |
|
|
T711 |
GCTCATGA |
GCTCATGA |
|
|
T712 |
ATCTCAGG |
ATCTCAGG |
|
|
T713 |
ACTCGCTA |
ACTCGCTA |
|
|
T714 |
GGAGCTAC |
GGAGCTAC |
|
|
T715 |
GCGTAGTA |
GCGTAGTA |
|
|
T716 |
CGGAGCCT |
CGGAGCCT |
|
|
T717 |
TACGCTGC |
TACGCTGC |
|
|
T718 |
ATGCGCAG |
ATGCGCAG |
|
|
T719 |
TAGCGCTC |
TAGCGCTC |
|
|
T720 |
ACTGAGCG |
ACTGAGCG |
|
|
T721 |
CCTAAGAC |
CCTAAGAC |
|
|
T722 |
CGATCAGT |
CGATCAGT |
|
|
T723 |
TGCAGCTA |
TGCAGCTA |
|
|
T724 |
TCGACGTC |
TCGACGTC |
|
Note: The usage volume of a single DNA library T5XX or T7XX is 1 μL
Notes
1. For your safety and health, please wear lab coats and disposable gloves when operating.
2. Please fill in the sequence of T5XX Index according to the corresponding sequencing platform and sequencing reagents.
3. Be careful to prevent crosstalk between different Indexes.
4. This product is for scientific research purposes only!
Sequence information
The DNA library structure constructed using the NGS Tagment Index Kit for Illumina is as follows:
The kit contains 16 types of T5XX and 24 types of T7XX, which can be combined into 384 different double-end index combinations for distinguishing different samples during high-throughput sequencing. The index sequences are shown in the following table:
|
Name |
Sequence |
|
T5XX |
5'-AATGATACGGCGACCACCGAGATCTACAC[IIIIIIII]TCGTCGGCAGCGTC-3' |
|
T7XX |
5'-CAAGCAGAAGACGGCATACGAGAT[IIIIIIII]GTCTCGTGGGCTCGG-3' |
Note: IIIIIIIII represents an 8 bp Index sequence. Before sequencing, enter the Index sequence used in the Sample Sheet according to the sequencing platform.