One Step RT-qPCR probe kit
Description
One Step qRT-PCR Probe Kit is specially designed for qPCR that directly use RNA (e.g. virus RNA) as template. Using gene specific primers (GSP), the reverse transcription and qPCR can be finished in one tube, significantly reducing pipetting procedures and the risk of contamination. It can be inactivated at 55℃ without affecting the efficiency and sensitivity of qRT-PCR. One Step qRT-PCR Probe Kit is provided in Master Mix. The 5 × One Step Mix contains an optimized buffer and dNTP/dUTP Mix, and is suitable for high-specificity detection systems based on fluorescence labelled probes (e.g. TaqMan).
Basic Principles of RT-qPCR
Speficification
|
Test Items |
Specification |
Result |
|
(SDS PAGE) Purity of Enzyme Stock(SDS PAGE) |
≥95% |
Pass |
|
Endonuclease Activity |
Not detected |
Pass |
|
Exodulease Activity |
Not detected |
Pass |
|
Rnase Activity |
Not detected |
Pass |
|
Residual E.coli DNA |
＜1copies/60 |
Pass |
|
Functional Assay-System |
90%≤110% |
Pass |
Components
|
Components |
100rxns |
1,000rns |
5,000 rxns |
|
RNase-free ddH2O |
2*1ml |
20ml |
100ml |
|
5*one step mix |
600μl |
6*1ml |
30ml |
|
One step enzyme mix |
150μl |
2*750μl |
7.5ml |
|
50* ROX reference Dye 1 |
60μl |
600μl |
3*1ml |
|
50* ROX reference Dye 2 |
60μl |
600μl |
3*1ml |
a. One-Step Buffer includes dNTP Mix and Mg2+.
b. Enzyme Mix mainly contains reverse
transcriptase, Hot Start Taq DNA polymerase (antibody modification) and RNase inhibitor.
c. Used to correct the error of fluorescene sinals between diferent wells.
d. ROX: You need to choose the calibration according to the model of the testing instrument.
Applications
QPCR detection
Shipping and Storage
Shipping: Ice packs
Storage Conditions: Store at -20℃.
Shief Life: 18months
