Plasmid Residual DNA Detection Kit HYI125
HYASEN® Plasmid Residual DNA assay Kit is used to quantitate plasmid DNA residues in gene therapy products, such as the plasmid DNA of lentiviral vector preparation in CAR-T cell therapy. This kit uses duplex real-time PCR technology to detect trace amounts of residual plasmid DNA by means of consensual plasmid sequences, such as replicon of ColE1/pMB1/pBR322/pUC. The target gene (FAM) performs rapid, specific, and reliable quantitative determination of the 102 copies/μL level of residual Plasmid DNA. IPC—Internal Positive Control (VIC) is included in the Plasmid Primer&Probe MIX to evaluate the performance of each PCR reaction. Customers can send DNA sequence of empty plasmid backbones to our technicians for confirmation in advance. For extraction information, please refer to the HYASEN® Residual Host Cell DNA Sample Preparation Kit (Cat. No.: HYI126).
Components & Storage
|
Reagent |
Quantity |
Storage |
|
Plasmid linear DNA Control |
lyophilized powder ×1 tube |
-20℃ |
|
Plasmid Primer&Probe MIX (Incl IPC) |
500 μL × 1 tube |
-20℃ protect form light |
|
qPCR Master MIX |
850 μL × 2 tubes |
-20℃ protect form light |
|
DNA Dilution Buffer (DDB) |
1.5 mL × 3 tubes |
-20℃ |
The kit components can be stored at appropriate conditions for up to 24 months. Please check the expiration date on the labels.
Applied instruments, including but not limited to the following
1.7500Real-Time PCR System
2.CFX96Real-Time PCR System
3.Lightcycler480Real-Time PCR System
Required materials not included in the kit
1.Nonstick, DNase-free&Low Retention Microfuge Tubes, 1.5mL
2.Nonstick, Low Retention Tips, 1000μL, 100μL, 10μL
3.96-well qPCR plates or PCR 8-strip tubes
Related equipment
1.Real-Time PCR System
2.Vortex mixer
3.Micro plate shaker
4.Pipettes, 1000μL, 100μL, 10μL
Workflow
