NGS DNA Library Prep Primer Mix for Illumina HYD313
NGS DNA Library Prep Primer Mix for Illumina is a special kit designed for DNA library preparation of Illumina platforms, using with Complete Adapter for Illumina or other equivalent products. All the reagents provided in the kit are subjected strict quality control and functional verification to ensure the stability and repeatability of the library preparation.
Components
|
Components |
8 T |
24T |
96T |
|
NGS DNA Library Prep Primer Mix for Illumina |
40 μL |
120 μL |
480 μL |
Storage
Store at -25~-15℃ for 1 year.
Specifications
|
Library |
DNA Library |
|
Input DNA |
100pg-1μg |
|
Time for library preparation |
~3 h |
|
Sequencing platforms |
Illumina |
|
Applications |
Whole genome shotgun sequencing, targeted sequencing by solution hybrid selection |
Instructions
|
Components |
Volume |
|
Canace™ Pro Amplification Mix |
25 μL |
|
NGS DNA Library Prep Primer Mix for Illumina |
5 μL |
|
Adapter Ligated DNA |
20 μL |
According to the library preparation manual prepare the reaction system (as shown in the table) and set the procedure.
Notes
1. For your safety and health, please wear lab coats and disposable gloves for operation.
2. Thaw components at room temperature. After thawing, mix thoroughly by vortexing, spin the tube briefly andplace them on ice for later use.
3. lt is recommended to pipette or gently shake during the reaction solution preparation of each step. Vigorousshaking may cause a decrease in library output.
4. It is highly recommended to use filtered pipet tips to avoid cross-contamination. Be sure to change pipet tipswhen processing different samples.
5. It is recommended to perform each reaction step in a thermocycler with a heated lid. The thermocycler shouldbe preheated to the set temperature before use.
6. Improper operations may very likely cause carry-over contaminations through aerosols, impacting theexperiments accuracy. It is highly recommended to divide the experiment environment into the pre-PCR and post-PCR regions, with separate sets of devices and disposables in each area. Perform routine cleaning for each area by wiping the surfaces with 0.5% sodium hypochlorite or 10% bleach.
7. This product is used for scientific research purposes only!