Product Overview
dNTP Mix (25 mM each) for mNGS is a high-purity nucleotide mixture developed for metagenomic next-generation sequencing (mNGS) and other sensitive nucleic acid amplification applications requiring stringent control of background DNA contamination. The solution contains four deoxyribonucleoside triphosphates—dATP, dCTP, dGTP, and dTTP—each at a concentration of 25 mM, with an HPLC purity of ≥99% for each component. In sensitive sequencing workflows, particularly pathogen detection and microbial profiling, trace amounts of contaminating DNA introduced by reagents may interfere with downstream analysis.
To address this challenge, Hyasen’s dNTP Mix for mNGS is manufactured with stringent quality control procedures to minimize residual DNA contamination and ensure consistent reagent quality. The formulation is quality-controlled for unwanted DNase, RNase, and phosphatase activities, as well as substances that may inhibit enzymatic amplification reactions. With its concentrated, ready-to-use formulation, the product allows flexible reaction setup and convenient integration into PCR-based amplification, NGS library preparation, and OEM master mix manufacturing. dNTP Mix (25 mM each) for mNGS provides a reliable nucleotide source for molecular diagnostics research, metagenomic sequencing sample preparation, and high-sensitivity nucleic acid amplification workflows.
Specifications
| Product name | Size-1 | Size-2 | Size-3 |
| dNTP mix (25 mM each) mNGS | 1 mL | 5 × 1 mL | 100 mL |
Product Features
- Stringent DNA Contamination Control: Manufactured under stringent quality control procedures to minimize residual DNA contamination, making it suitable for sensitive mNGS and nucleic acid amplification workflows.
- High Nucleotide Purity: Contains dATP, dCTP, dGTP, and dTTP, each with an HPLC purity of ≥99%, supporting consistent performance in enzymatic reactions.
- Quality-Controlled Enzymatic Contaminants: Tested for unwanted DNase, RNase, and phosphatase activities to support reagent integrity and downstream amplification performance.
- Concentrated and Ready-to-Use Formulation: Each dNTP is supplied at 25 mM, allowing smaller addition volumes and greater flexibility in reaction formulation.
- Suitable for OEM and Molecular Diagnostic Reagent Manufacturing: Available in multiple packaging sizes for laboratory applications, reagent formulation, and bulk manufacturing requirements.
FAQ
Q1: What is the concentration of dNTP Mix (25 mM each)?
The mixture contains dATP, dCTP, dGTP, and dTTP, each at 25 mM, giving a total dNTP concentration of 100 mM. For example, adding 0.4 µL of the mix to a final reaction volume of 50 µL provides 200 µM of each dNTP. The optimal concentration should be determined according to the specific application and enzyme system.
Q2: What makes this dNTP Mix suitable for mNGS applications?
Metagenomic sequencing workflows can be highly sensitive to background DNA contamination, particularly when analyzing low-biomass samples. Hyasen’s dNTP Mix for mNGS is manufactured with stringent DNA contamination control and quality testing to minimize the introduction of unwanted nucleic acids during sample preparation and amplification.
Q3: Can this product be used for PCR, qPCR, and NGS library preparation?
The product can serve as a nucleotide substrate for DNA polymerase-based applications, including PCR, qPCR, and amplification steps in NGS library preparation. Reaction conditions and product compatibility should be validated for each specific workflow.
Q4: Is this dNTP Mix available for OEM manufacturing?
Yes. Hyasen offers multiple packaging sizes, including 1 mL, 5 × 1 mL, and 100 mL, to support research, reagent formulation, and OEM manufacturing requirements. Please contact our team for technical specifications, quality documentation, and bulk supply inquiries.
