Product Overview
Library preparation is a repetitive, multi-step part of RNA sequencing, and each manual pipetting step is a chance for variation. For laboratories running many samples, reducing the number of reagent components and the amount of hands-on work improves both throughput and reproducibility. The Hyaseq RNA Library Prep Kit is designed for total RNA sequencing library preparation and is compatible with both Illumina and MGI platforms. The kit includes reagents for RNA fragmentation, reverse transcription, ds-cDNA synthesis, and library amplification, and it can be combined with mRNA purification or rRNA depletion kits, so it fits into different RNA-seq workflows. It is available in two formats: a standard tube format, and a pre-loaded plate format. The plate format removes manual preparation and sub-packaging, and its tear-and-use design allows direct integration with automated library construction systems, which suits high-throughput laboratories and service providers. Enzymes and buffers are pre-mixed to reduce the number of reagent components and simplify preparation, and the reagents need no additional light protection during handling. All components undergo rigorous quality control and functional validation to ensure stable, reproducible library preparation results.
Product Features
- Illumina and MGI platform compatibility: One kit supports total RNA sequencing library preparation for both Illumina and MGI platforms, so laboratories running both do not need separate library prep products.
- Pre-loaded plate format for automation: The pre-loaded plate removes manual preparation and sub-packaging and works with a tear-and-use operation, allowing direct integration with automated library construction instruments.
- Premixed reagents with validated quality: Enzymes and buffers are pre-mixed to reduce reagent components and simplify setup. All components undergo rigorous quality control and functional validation to support stable, reproducible results.
FAQ
Q1: Which sequencing platforms is this kit compatible with?
The kit is designed for total RNA sequencing library preparation and is compatible with both Illumina and MGI platforms.
Q2: What is the difference between the standard tube format and the pre-loaded plate format?
Both formats are designed for the same library preparation workflow. The standard tube format is supplied in conventional tubes. The pre-loaded plate format comes with reagents already dispensed into a plate, which removes manual preparation and sub-packaging and allows tear-and-use operation. This format is designed for direct integration with automated library construction instruments, so it suits high-throughput workflows.
Q3: Which workflow steps does the kit cover, and can it be used with rRNA depletion or mRNA purification?
The kit includes reagents for RNA fragmentation, reverse transcription, ds-cDNA synthesis, and library amplification. It can be combined with mRNA purification or rRNA depletion kits, so the upstream RNA enrichment step can be chosen to match your sample type and sequencing goal.
