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Proteinase K( Lyophilized powder) Featured Image
  • Proteinase K( Lyophilized powder)
  • Proteinase K( Lyophilized powder)

Proteinase K( Lyophilized powder)


CAS no.: 39450-01-6

EC no.: 3.4.21.64

Package: 1g, 10g, 100g

Product Detail

Advantages

● Higher stability and enzyme activity based on directed evolution technologies

● Tolerant of Guanidine salt

● RNase free, DNase free and Nickase free,DNA <5 pg/mg 

Description

Proteinase K is a stable serine protease with broad substrate specificity. It degrades many proteins in the native state even in the presence of detergents. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active site catalytic triad (Asp 39-His 69-Ser 224). The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity.

Chemical structure

Chemical structure

Specification

Test items

Specifications

Description

White to off white amorphous powder, Lyophilied

Activity

≥30U/mg

Solubility(50mg Powder/mL)

Clear

RNase 

None detected

DNase

None detected

Nickase

None detected

Applications

Genetic diagnostic kit;

RNA and DNA extraction kits;

Extraction of non-protein components from tissues,degradation of protein impurities, such as

DNA vaccines and preparation of heparin;

Preparation of chromosome DNA by pulsed electrophoresis;  

Western blot;

Enzymatic glycosylated albumin reagents in vitro diagnostics

Shipping and Storage

Shipping: Ambient

Storage Conditions:  Store at -20℃(Long term)/ 2-8℃( Short term)

Recommended re-test date: 2Years

Precautions

Wear protective gloves and goggles when using or weighing, and keep well ventilated after use. This product may cause skin allergic reaction. Cause serious eye irritation. If inhaled, it may cause allergy or asthma symptoms or dyspnea. May cause respiratory irritation.

Assay Unit Definition

One unit (U) is defined as the amount of enzyme required to hydrolyze casein to produce 1 μmol tyrosine per minute under the following conditions.


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