Proteinase K（Lyophilized powder）HYC110
Proteinase K is a stable serine protease with broad substrate specificity. It degrades many proteins in the native state even in the presence of detergents. Evidence from crystal and molecular structure studies indicates the enzyme belongs to the subtilisin family with an active site catalytic triad (Asp39-His69- Ser224). The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. It is commonly used for its broad specificity.
Components
|
Components |
Size-1 |
Size-2 |
Size-3 |
Size-4 |
|
Proteinase K（Lyophilized powder） |
0.1g |
1g |
10g |
100g |
Storage
Dry powder status -25～-15℃ valid for 36 months; the enzyme powder should be dissolved into the appropriate volume, 2-8℃ short-term storage away from light, -25～-15℃ long-term storage away from light.
Specification
|
Appearance |
White to off-white amorphous lyophilized powder |
|
Specific Activity |
≥30 U/mg |
|
DNase |
None detected |
|
RNase |
None detected |
|
Nickase |
None detected |
Properties
|
Source |
Recombinant from Tritirachium album |
|
EC number |
|
|
Molecular weight |
29 kDa (SDS - PAGE) |
|
Isoelectric point |
7.81 |
|
Optimum pH |
7.0- 12.0 |
|
Optimum temperature |
65 ℃ |
|
pH stability |
pH 4.5- 12.5 (25 ℃, 16 h) |
|
Thermal stability |
Below 50 ℃ (pH 8.0,30 min) |
|
Storage stability |
Over 90% activity stored at 25℃ for 12months |
|
Activators |
SDS, Urea |
|
Inhibitors |
Diisopropyl fluorophosphate（DFP） |
Unit definition
One unit (U) of Proteinase K is defined as the amount of enzyme required to hydrolyze casein to produce 1 μmol tyrosine per minute under standard determination conditions.
Applications
Genetic diagnostic kit; RNA and DNA extraction kits; Extraction of non-protein components from tissues, degradation of protein impurities, such as DNA vaccines and preparation of heparin; Preparation of chromosome DNA by pulsed electrophoresis; Western blot; Enzymatic glycosylated albumin reagents in vitro diagnosis.
Notes
Wear protective gloves and goggles when using or weighing, and keep well ventilated after use. This product may cause skin allergic reaction and serious eye irritation. If inhaled, it may cause allergy or asthma symptoms or dyspnea. May cause respiratory irritation.