Rapid DNA Lib Prep Kit V2 HYD114
Rapid DNA Lib Prep Kit V2 contains the enzymes and buffers required to convert abroad range of input amounts of DNA into high quality libraries for next-generation sequencing on the Illumina® NGS platforms.Inputs of 1 ng to 1000 ng double-stranded DNA(dsDNA) are required for library preparation. The entire four-step work flow takes place in a single tube or well, in about three hours(Figure 1). No intermediate purification steps or sample transfers are necessary, thus preventing handling errors and loss of valuable samples. Once purified and quantified, the resulting libraries are ready for Illumina NGS instruments using standard Illumina sequencing reagents and protocols. The kit provides excellent results for high-coverage deep sequencing, such as de novo sequencing, whole genome resequencing, whole exome sequencing, and other enrichment techniques.
Reagent Composition
|
Components |
24T |
96T |
|
|
End Preparation |
End Prep Buffer II |
168 μL |
672μL |
|
End Prep Enzymes |
72 μL |
288μL |
|
|
Adapter Ligation |
Ligation Buffer |
720 μL |
2880μL |
|
Ligase Enzymes II |
240 μL |
960μL |
|
|
Amplification |
2X PCR Mix |
600 μL |
2400μL |
|
10X ILM PCR Primers |
120 μL |
480 μL |
|
|
MGI PCR Primer Mix |
120 μL |
480 μL |
Storage Conditions
All components should be stored at -20°C. The shelf life of each reagent is one year when stored properly.
Notes
Make sure to use high-quality DNA samples. Heavily nicked or damaged DNA will significantly lower library yield. We recommend Qubit® or other fluorometric methods to quantify DNA input. Residual trace RNA, nucleotides, ssDNAor other contaminants will decrease the efficiency of enzymes for optimized library preparation.