N-Glycan Kit (single column) HYI141
This document provides information regarding the general care and use of N-Glycan Kit for the fast enzymatic release and rapid labeling of N-glycans. This protocol is validated using monoclonal antibodies and has also been tested to perform for a wide range of other N-linked glycoproteins. We recommend the user to confirm enzymatic release for their particular sample.
Components
|
Module |
Reagent |
5 T |
|
Deglycosylation Module |
IgG |
For users to trouble shooting and process validation |
|
PNGase F |
For rapid deglycosylation |
|
|
PNGase F buffer |
For rapid deglycosylation |
|
|
Surfactant |
enzyme-friendly surfactant used to denature the glycoproteins |
|
|
Labeling Module |
MS reagent powder |
For samples Labeling |
|
Anhydrous DMF |
As the resolution reagent of label |
|
|
Clean-up Module |
SPE column |
For removing potential interferences |
|
Elution Buffer |
For Optimum glycan recovery |
|
|
Sample Diluent |
For diluting SPE eluste |
Transportation and storage
|
Reagent |
Storage |
|
IgG |
2-8℃, 12 months |
|
PNGase F |
-25~-15℃℃ , 12 months , avoid freeze/thaw cycles |
|
PNGase F buffer |
2-8℃, 12 months |
|
Surfactant |
Room Temperature, 12 months |
|
MS reagent Powder |
2-8℃, 12 months,keep in dark |
|
Anhydrous DMF |
Room temperature, 12 months |
|
Elution Buffer |
Room temperature, 12 months |
|
Sample diluent |
Room temperature, 12 months |
|
SPE column |
Room temperature, once opened, the unused well need to be sealed. |