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Reverse Transcriptase, Glycerol-free Featured Image
  • Reverse Transcriptase, Glycerol-free

Reverse Transcriptase, Glycerol-free


CAS no.:9068-38-6    EC no.:2.7.7.49

Package: 10000U, 40000U.

Product Description

Description

Reverse transcriptase 200U/μL,Glycerol-frees is a new reverse transcriptase obtained through genetic engineering technology. Compared with M-MLV () Reverse Transcriptase, its thermal stability It is greatly improved and can withstand reaction temperatures up to 65°C, suitable for reverse transcription of RNA templates with complex secondary structures. At the same time, the enzyme has enhanced affinity with the template, which is suitable for the reverse transcription of a small amount of template and low-copy genes. The ability of Reverse Transcriptase to synthesize full-length cDNA has also been improved, and cDNA up to 19.8 kb can be amplified. Reverse Transcriptase, 200 U/μL, Glycerol-free third-generation thermostable reverse transcriptase (glycerol-free version) can be used to prepare lyophilized preparations, lyophilized RT-LAMP reagents, etc.

Chemical structure

Chemical structure6

Specification

Test Items

 Specification

Result

(SDS PAGE) Purity of Enzyme Stock(SDS PAGE)

≥95%

Pass

Endonuclease Activity

Not detected

Pass

Exodulease Activity

Not detected

Pass

Rnase Activity

Not detected

Pass

Residual E.coli DNA

<1 copy/60U

Pass

Functional Assay-System

90%≤110%

Pass

Applications

Freeze-dried Kit

Lyophilizable RT-LAMP kit.

Shipping and Storage

Transportation: Ice packs

Storage Conditions: Store at -30~-15℃.

Shief life: 18Months


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