RNase assay Kit(Fluorescence) HYJ526
The RNase detection kit is based on a fluorophore-labeled RNA probe. When the sample does not contain RNase activity, the probe is stable and does not produce a fluorescent signal; when the sample contains RNase activity, the probe is degraded, resulting in a gradual enhanced fluorescence signal; the rate of increase in fluorescence signal is positively correlated with the number and activity of enzymes. Use a fluorescence microplate reader to measure at the wavelength of ex/em=535/575nm to determine whether the sample is contaminated by RNase.
Components
|
Components |
48 T |
192 T |
|
10×reaction solution |
0.5mL |
2.0mL |
|
RNA probe |
1tube |
1tube |
|
TE buffer |
0.5mL |
2.0mL |
|
RNase A standard (10mg/mL) |
10μL |
20μL |
|
Standard Dilution Buffer |
6mL |
12mL |
|
DNase＆RNase-free water |
25mL |
25mL |
|
DNase RNase away |
50mL |
50mL |
Storage
1. Transported in -25 ~ -15℃;
2. The different components of the kit are storedseparately according to the temperature：
|
Components |
Temperature |
|
10×reaction solution |
-25~-15℃ |
|
RNA probe |
-25~-15℃ |
|
TE buffer |
-25~-15℃ |
|
RNase A standard (10mg/mL) |
-25~-15℃ |
|
Standard Dilution Buffer |
-25~-15℃ |
|
DNase＆RNase-free water |
-25~30℃ |
|
DNase RNase away |
2~30℃ |
3. Store the unopened kit for 12 months.
4. Store the kit for 6 months after opening. It isrecommended to aliquot the RNA probe solution according to the single use amount to avoid light and repeated freezing and thawing
Additional Materials Required
1. Fluorescence microplate reader (includingex/em=535/575nm wavelength)
2. DNase＆RNase-free pipettes and tips
3. DNase＆RNase-free EP tube
4. DNase＆RNase-free black non-transparent96-well plate