RNase III--HYJ235
This product is from an E. coli strain containing a genetic fusion of the E. coli RNase III gene (rnc) and the gene coding for maltose binding protein (MBP). This specific exonuclease cleaves double-stranded RNA (dsRNA) and generates 18-25bp dsRNA fragments with 5'-PO4 and 3'-OH, 3' overhangs.
Components
|
Components |
0.2KU |
1KU |
|
RNase III（2U/μL） |
100 uL |
500 uL |
|
10 × Buffer |
1mL |
1mL |
|
10 × EDTA |
1mL |
1mL |
|
MnCI2 |
1mL |
1mL |
|
Glycogen RNase-Free |
0.05mL |
0.05mL |
Storage
Store at -25～-15℃, transportation by dry ice.
Unit definition
One unit refers to the amount of enzyme required to degrade 1 μg of dsRNA to siRNA in a 50 μL reaction system at 37°C for 20 minutes. (Optimum reaction temperature: 37℃)
Quality control
Purity≥95%, no DNase, RNase activity, no Exonuclease Activity
Reaction System and Condition
|
Components |
Volume |
|
Nuclease-free Water |
Up to 100 uL |
|
10 × Buffer |
10uL |
|
dsRNA |
10ug |
|
RNase III |
10uL |
|
10 × MnCI2 |
10uL |
Reaction time: Incubate at 37 °C for 1 h.
Reaction Inactivation:Add 10 µl 10 × EDTA to stop the reaction. Do not heat inactivate. Heat
inactivation reduces the yield of siRNA.
Notes
- For your safety and health, please wear a lab coat and disposable gloves.
- The volume of the reaction system can be scaled up or down according to actual needs.