Rnase R HYC123
Ribonuclease R from E. coli, is a magnesium-dependent 3 ′ → 5 ′ exoribonuclease that can digest alllinear RNAs but does not digest lariat or circular RNA structures. Most cellular RNAs will be digested completely by RNase R, with the exception of tRNAs, 5S RNA and intron lariats. RNase R is used for enrichment of circRNA sin biological samples, identification of intronic lariat sequences, identification of exonic circRNAs, studying alternative splicing.
Storage Conditions
The product can be stored at -25～- 15 ℃ for 2 years
Instructions
This Please prepare the following reaction system in a sterile micro-centrifuge tube;
Reaction System
|
Components |
Volume |
|
10* Rnase R Reaction Buffer |
2μL |
|
RNA sample |
1 μg |
|
Rnase R 20 (u/μL） |
2-4 U |
|
Rnase-free ddH2O |
Up to 20 μL |
Incubation at 37℃ for 10-30 minutes
Notes
1. The activity of RNase R requires 0.1-1.0 mM Mg2+;
2. With the increase of RNA, the reaction time can be extended and the enzyme amount can be increased appropriately;
3. The EDTA content in RNA samples may affect the activity of RNase R.
4. Incubate at 70℃ for 10 minutes can inactivate the enzyme.