RT-LAMP Probe Master Mix (Lyophilized beads） HYB416
This product contains reaction buffer, Bst DNA polymerase and heat-resistant reverse transcriptase RT-Enzymes Mix, and freeze-dried protectant components. The reaction buffer contains Mg2+, dNTP and other necessary components for amplification. The product is in the form of freeze-dried pellets, and only primer probes and templates need to be added when using it.
Components
|
Components |
96 T |
960 T |
9600 T |
|
RT-LAMP Probe Master Mix (Lyophilized beads） |
8 T/strip×12 |
8 T/strip×120 |
8 T/strip×1200 |
Storage
Store at 2-8℃, valid for 12 months.
Instructions
1. Take out the corresponding number of Lyophilized beads according to the number of tests.
2. Prepare the reaction system
|
Component |
Volume |
|
RT-LAMP Probe Master Mix (Lyophilized beads） |
One piece |
|
10×Primer probe Mix* |
5 μL |
|
Template RNA** |
45 μL |
*10× Primer Mix concentration: 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B，4 μM probe;
**It is recommended to dissolve the nucleic acid template in DEPC water.
3. Amplification reaction
Set up the following program on a fluorescent PCR instrument (such as ABI7500, etc.).
|
Temperature |
Time |
Cycles |
|
65℃ |
60s *Collect FAM fluorescence |
30 |
Notes
1. The reaction temperature can be optimized between 62℃-68℃ according to the primer situation;
2. The experiment should be operated in a standardized manner, including the preparation of the reaction system, freeze-drying, sample processing and sample addition;
3. To avoid contamination, it is recommended to prepare the reaction system in a clean bench and add the template in a fume hood in other rooms to avoid false positive interference.