Kit ya ADN/RNA ya Virusi ya Magnetic Universal Viral HYD521
Kit ya Magnetic Universal Viral DNA/RNA Kit ikwiriye gukura aside nucleic ya virusi mu maraso yose cyangwa mu mazi adafite uturemangingo (nk'amaraso, plasma, amazi yo mu bwonko, amazi yo mu mazuru/mu nda, amazi yo koza alveolar, nibindi). Ubu buryo bukoresha ikoranabuhanga ryo gutunganya amasaro ya magnetic, kandi nta gusohora phenol chloroform irimo uburozi bikenewe mu gihe cyo kuyikuramo. Ni nziza, ntigira uburozi kandi ni vuba. Umusaruro wabonetse ushobora gukoreshwa mu buryo butaziguye muri PCR, qPCR, sequencing y'ikinyejana cya kabiri n'ibindi bipimo. Iyo ikoreshejwe n'ibikoresho byo gukuramo amasaro ya magnetic, gusohora aside nucleic ya magnetic kwagutse cyane.
Amakuru y'ibicuruzwa
| Ibice bigize | 48T |
| Umuti wa Lysis na Binding | mililitiro 33 |
| Udupira tw'amasaro dufite ikoranabuhanga rya magnetique | mililitiro 1.1 |
| Akabati ko gukaraba A | mililitiro 40 |
| Akabati ko gukaraba B | mililitiro 80 |
| Igisubizo cya Elution | mililitiro 10 |
| Poroteyine K | mililitiro 1.1 |
Ibisabwa mu kubika
Proteyine K igomba kubikwa ku bushyuhe bwa 2 ~ 8°C naho ibindi bikoresho bikabikwa ku bushyuhe bw'icyumba. Igihe cyo kubika ni amezi 18.
Ibisobanuro by'icyitegererezo
1. Ubwoko bw'ibipimo bikoreshwa: amaraso yose, serum, plasma, amazi yo mu bwonko, amazi yo mu mazuru/mu gifu, amazi yo koza mu maraso n'ibindi bipimo.
2. Kubika no gutwara ingero: Ingero zishobora gukoreshwa mu gupima ako kanya cyangwa zikabikwa kuri -70°C cyangwa munsi yayo kugira ngo zipimwe. Igihe cyo kubika ni amezi 6. Gukonjesha no gushonga kenshi bigomba kwirindwa. Ingero zitwarwa n'umugozi ukonje.
3. Ibisabwa mu gukonjesha no gushonga: Gukonjesha no gushonga vuba, wirinde gukonjesha no gushonga kenshi.
Igikorwa
Mbere y'igerageza, reba niba umuti ufite imvura kandi niba amasaro ya rukuruzi ashobora kongera gushyirwamo.
1. Shyira 200 ~ 300 μL y'icyitegererezo mu muyoboro wa centrifuge wa 1.5 mL, ongeramo 600 μL ya Lysis na Binding Solution, 20 μL ya Proteinase K, na 20 μL ya magnetic bead suspension mu buryo bukurikiranye, hanyuma ushyiremo vortex ku muvuduko mwinshi mu gihe cy'amasegonda 30. Shyira kuri 50℃ mu gihe cy'iminota 5 ~ 7. Niba thermostat idafite imikorere yo guhindagurika, shyiramo vortex inshuro 3 mu gihe cyo gushyuha, buri gihe mu gihe cy'amasegonda 15.
2. Shyira ku gikoresho cya magnetiki cya 1.5 mL kugira ngo gitandukanye kugeza igihe umuti uboneye kandi ubonerana, hanyuma ujugunye umuti.
3. Shyiramo 700 μL ya wash buffer A, vortex mu masegonda 10 kugira ngo uce uduce twa magnetic, hanyuma ushyire ku gitereko cya magnetic kugira ngo gitandukanye na magnetic kugeza igihe umuti uboneye, hanyuma ujugunye umuti.
4. Ongeraho 700 μL ya wash buffer B, vortex mu gihe cy'amasegonda 10 kugira ngo uce uduce twa magnetic, wimure ku gitereko cya magnetic kugira ngo utandukanye magnetic kugeza igihe umuti uvuyemo, hanyuma ujugunye umuti.
5. Ongera wongere 700 μL ya wash buffer B, ushyiremo vortex mu gihe cy'amasegonda 10 kugira ngo uce uduce twa magnetic, ushyire kuri magnetic stand kugira ngo utandukanye magnetic kugeza igihe umuti uvuyemo, hanyuma ujugunye umuti.
6. Shyira icyuma gishyushya amazi mu buryo bw'ingufu mu gihe gito kugira ngo uhuze ibitonyanga ku rukuta rw'umuyoboro, wimure ku gitereko cy'amashanyarazi kugira ngo ubone neza, hanyuma unyure amazi asigaye. Shyira umwuka mu gihe cy'iminota 3-5.
Icyitonderwa: Ibisigazwa bya Ethanol bizabuza imikorere ya enzymes zikurikiraho, bityo rero menya neza ko ethanol ishira burundu iyo yumye. Ntukamare igihe kirekire kugira ngo wirinde kugira ingaruka ku ngaruka za nyuma zo kuyikuraho.
7. Ongeraho 40~100 μL y'umuti wa Elution, ushyiremo vortex ku muvuduko wo hejuru mu gihe cy'iminota 2~3 kugira ngo ucemo uduce twa rukuruzi. Shyira ku bushyuhe bwa 60℃ mu minota 5, hanyuma ushyiremo vortex ku muvuduko wo hejuru mu minota 60.
8. Shyira amazi mu buryo bwihuse kugira ngo ufate amazi mu mupfundikizo w'umuyoboro hanyuma uyashyire mu gikoresho cy'amashanyarazi kugeza igihe amasaro ya rukuruzi arangije kwinjizwamo. Hanyuma wimure witonze amazi mu muyoboro mushya w'amashanyarazi kugira ngo ubone umuti wa aside nucleique.
9. Umuti wa aside nucleike ushobora kubikwa kuri -20°C kugira ngo ubibike igihe gito na -80°C kugira ngo bibikwe igihe kirekire.
Inyandiko
1. Udukoresho dutandukanye two muri aka gakoresho turimo umunyu wa guanidine. Kugira ngo ugire umutekano n'ubuzima bwiza, mbara ikoti rya laboratwari n'uturindantoki dukoreshwa rimwe. Kandi ufate ukurikije amabwiriza y'umutekano. Ntureke ngo agakoresho kagere ku ruhu, amaso n'uturemangingo tw'imitsi. Niba bibaye, banza woge vuba n'amazi menshi kandi ushake ubuvuzi.
2. Iyo umuti uguye, ugomba kogezwa mu mazi afite ubushyuhe bwa 30°C kugeza igihe amazi aguye burundu mbere yo kuwukoresha.
3. Niba icyuma gikingira urusaku cyakonjeshejwe, ntugikoreshe.
4. Udukoresho dutandukanye two muri aka gakoresho turimo umunyu wa guanidine. Ntugakoreshe imiti yica udukoko nka sodium hypochlorite mu kuvura, bitabaye ibyo imyuka ihumanya izavamo kandi igomba gufatwa nk'imyanda yo kwa muganga.
5. Hashobora kubaho udusaro twa rukuruzi dusigaye mu gihe cyo kuvura. Gerageza kwirinda guhumeka udusaro twa rukuruzi mu gihe cyo kuvura ingero.