Sample Preparation Kit for mRNA Capping Efficiency Detection HYJ511
Capping efficiency is a critical quality attribute for mRNA-based vaccines, because a cap is necessary at the 5’end of the mRNA molecule to protect the molecule from degradation and to facilitate successful protein translation. The Sample Preparation Kit for mRNA Capping Efficiency Detection enables efficient and reproducible processing of mRNA samples for accurate detection and quantitative assessment of ribose-methylated and ribose-unmethylated cap structures, which can be separated and quantified using LC-MS. This kit includes pre-formulated 4× RNase H Mix, Streptavidin Magnetic Beads, and an optimized protocol, enabling the preparation of LC-MS-ready samples within 1.5 hours.
Components
|
Components |
10 T |
50 T |
|
4×RNase H Mix |
250 μL |
5×250μL |
|
Streptavidin Magnetic Beads |
500 μL |
5×500μL |
|
Wash Buffer |
8×1 ml |
40×1 ml |
|
Elution Buffer |
300 μL |
5×300 μL |
|
mRNA Positive control（1μg/μL） |
200 μL |
5×200 μL |
|
Biotin Probe Positive Control (10μM) |
100 μL |
5×100 μL |
Storage
Transportation under 0°C; Streptavidin Magnetic Beads and Wash Buffer can be stored at 2~8 °C, and the other components can be stored at -25～-15 °C.
Additional Materials Required
Consumables: 20 μL and 200 μL pipette tips, PCR tubes, 1.5 mL microcentrifuge tubes. All consumables should be nuclease-free.
Reagent: RNase H cleavage probe, nuclease-free water.