Sample Preparation Kit for mRNA Poly(A) Tail Length Detection HYJ512
Poly(A) tail length distribution is a critical quality attribute for mRNA-based vaccines, for it is necessary at the 3’ end of the mRNA molecule to protect the molecule from degradation and to facilitate successful protein translation. The Sample Preparation Kit for mRNA Poly(A) Tail Length Detection enables efficient and reproducible processing of mRNA samples for accurate detection and assessment of the poly(A) tail length distribution. This kit includes pre-formulated 10× RNase T1 Mix, resin, spin column, wash buffer and elution buffer, enabling the preparation of LC-MS-ready samples within 1 hours.
Components
|
Components |
10 T |
50 T |
|
10×RNase T1 Mix |
200 μL |
5×200μL |
|
mRNA for positive control (1μg/μL) |
100 μL |
5×100μL |
|
Wash buffer 1 |
9×1 ml |
45×1 ml |
|
Wash buffer 2 |
1 ml |
5×1 ml |
|
Elution buffer |
1 ml |
5×1 ml |
|
Resin |
200 μL |
5×200μL |
|
Spin columns |
10 pcs |
5×10 pcs |
Storage
Transportation under 0°C. Resin can be stored at 2~ 8 ℃, Spin column can be stored at room temperature, 10×RNaseT1 Mix, Wash buffer and Elution buffer can be stored at -25 ~ -15 ℃.
Additional Materials Required
Consumables: 20 μL and 200 μL pipette tips, 1.5 mL microcentrifuge tubes. All consumables should be nuclease-free.
Reagent: nuclease-free water.