Kit yeMagnetic Universal Viral DNA/RNA HYD521
Kit yeMagnetic Universal Viral DNA/RNA Kit yakakodzera kubvisa viral nucleic acid kubva muropa rose kana mvura yemuviri isina masero (senge serum, plasma, cerebrospinal fluid, nasal/pharyngeal swabs, alveolar lavage fluid, nezvimwewo). Nzira iyi inoshandisa tekinoroji yekuchenesa magnetic bead, uye hapana chepfu inobviswa phenol chloroform inodiwa panguva yekubvisa. Yakachengeteka, haina chepfu uye inokurumidza. Chigadzirwa chakawanikwa chinogona kushandiswa zvakananga muPCR, qPCR, second-generation sequencing nedzimwe nzira. Kana ikashandiswa ne magnetic bead automated extraction instruments, high-throughput extraction ye nucleic acids inogona kuwanikwa.
Ruzivo rwechigadzirwa
| Zvikamu | 48T |
| Lysis uye Kusunga Mhinduro | 33 mL |
| Kumiswa kwemagineti kwebead | 1.1 mL |
| Chivharo chekushambidza A | 40 mL |
| Chivharo chekushambidza B | 80 mL |
| Mhinduro yeElution | 10 mL |
| Mapuroteni K | 1.1 mL |
Mamiriro Ekuchengetedza
Proteinase K inofanira kuchengetwa pa2~8℃ uye zvimwe zvinoshandiswa zvinofanira kuchengetwa patembiricha yemumba. Nguva yekushanda kwayo imwedzi gumi nemisere.
Tsananguro yemuenzaniso
1. Mhando dzezviyero zvinoshanda: ropa rose, serum, plasma, cerebrospinal fluid, nasal/pharyngeal swabs, alveolar lavage fluid nedzimwe sampuro.
2. Kuchengetera nekutakura sampuli: Mienzaniso inogona kushandiswa kuongororwa ipapo ipapo kana kuchengetwa pa -70℃ kana pasi kuti iongororwe. Nguva yekuchengetedza imwedzi mitanhatu. Kuomesa nechando zvakare uye kunyunguduka kunofanira kudzivirirwa. Mienzaniso inotakurwa nechando.
3. Zvinodiwa kuti iome nechando: Inokurumidza kuomeswa nechando uye kunyungudika, dzivisa kuomeswa nechando uye kunyungudika kakawanda.
Kushanda
Usati waedza, tarisa kana mvura iri mumhinduro yacho ine mvura uye kana mabhora emagineti anogona kudzoserwa.
1. Tumira 200~300 μL yemuenzaniso muchubhu ye centrifuge ye 1.5 mL, wedzera 600 μL yeLysis and Binding Solution, 20 μL yeProteinase K, uye 20 μL yemagnetic bead suspension zvakatevedzana, uye vortex nekumhanya kwakanyanya kwemasekonzi 30. Ivai pa50℃ kwemaminitsi 5~7. Kana thermostat isina oscillation function, vortex katatu panguva ye incubation period, nguva imwe neimwe kwemasekonzi 15.
2. Isa mushonga we magnetic stand we 1.5 mL kuti uparadzanise magnetic stand kusvika wanyatsojeka, wobva warasa mushonga wacho.
3. Wedzera 700 μL ye wash buffer A, vortex kwemasekonzi gumi kuti uputse mabhora emagineti, wobva waisa panzvimbo yemagineti kuti uparadzanise magineti kusvika mhinduro yabuda, wobva warasa mhinduro yacho.
4. Wedzera 700 μL ye wash buffer B, vortex kwemasekonzi gumi kuti uputse magineti, isa panzvimbo yemagnetic kuti uparadzanise magineti kusvika mhinduro yabuda, wobva warasa mhinduro yacho.
5. Wedzera 700 μL yewash buffer B zvakare, bvisa vortex kwemasekonzi gumi kuti uputse magineti, isa panzvimbo yemagnetic kuti uparadzanise magineti kusvika mhinduro yabuda, wobva warasa mhinduro yacho.
6. Shandisa centrifuge kwenguva pfupi kuti uunganidze madonhwe pamadziro echubhu, woaisa panzvimbo ine magineti kuti anyatsojeka, wobva wanwisa mvura yasara. Omesa mhepo kwemaminitsi 3-5.
Cherechedza: Zvisaririra zveEthanol zvinodzivisa mafambiro ema enzyme anotevera, saka iva nechokwadi chekuti ethanol inopwa zvachose kana yaoma. Usaome kwenguva yakareba kuti usakanganisa mhedzisiro inotevera ye elution.
7. Wedzera 40~100 μL yeElution solution, vortex nekumhanya kwakanyanya kwemaminitsi 2~3 kuti uparadze magineti. Isai pa60℃ kwemaminitsi mashanu, mozoisa vortex nekumhanya kwakanyanya kwemaminitsi makumi matanhatu.
8. Shandisa centrifuge ipapo ipapo kuti uunganidze mvura kubva muchivharo chechubhu woiendesa kune rack yemagineti kusvika mabhora emagineti anyudzwa zvachose. Wobva waisa mvura yacho nokungwarira muchubhu itsva yemagineti kuti uwane mhinduro ye nucleic acid.
9. Mushonga we nucleic acid unogona kuchengetwa pa -20°C kuti uchengetwe kwenguva pfupi uye -80°C kuti uchengetwe kwenguva refu.
Zvinyorwa
1. Mabuffer akasiyana-siyana ari mukit iyi ane munyu weguanidine. Kuti muve vakachengeteka uye vane hutano, ndapota pfekai jasi rekurabhoritari uye magirovhosi anoraswa. Uye batai zvinoenderana nemutemo wekuchengetedza. Musarega buffer ichibata ganda, maziso uye mucous membranes. Kana zvikaitika, ndapota gezai nemvura yakawanda nekukurumidza motsvaga rubatsiro rwechiremba.
2. Kana mvura yacho ikaita seyakanyorova, inofanira kunyoroveswa mumvura ine tembiricha ye30℃ kusvika mvura yacho yanyungudika zvachose usati washandisa.
3. Kana simbi yemagnetic bead yakaomeswa nechando, usashandise.
4. Mabuffer akasiyana-siyana ari mukit iyi ane munyu weguanidine. Usashandise mishonga inouraya utachiona yakaita sesodium hypochlorite pakurapa, zvikasadaro magasi ane chepfu achaburitswa uye anofanira kubatwa semarara ekurapa.
5. Panogona kunge paine magineti akasara panguva yekubvisa magineti. Edza kudzivirira kufema magineti paunenge uchifema masampuli.