Spin Viral DNA/RNA Kit-HYC425
Its unique buffer system enables highly efficient and specific binding of viral nucleic acids in lysate to silica-membrane spin columns. The purified viral nucleic acids feature high purity and stable quality, free of proteins, nucleases and other contaminants, and are suitable for various routine applications including PCR and real-time PCR.
Components
|
Components |
50T |
|
Buffer RLC |
30 mL |
|
Buffer PGWT |
30 mL |
|
Buffer GWT2 |
30 mL |
|
Proteinase K |
1.25 mL |
|
RNase-Free Water |
10 mL |
|
Spin Columns DM with Collection Tubes |
50 |
|
RNase-Free Centrifuge Tubes (1.5 mL) |
50 |
Storage
All components can be stably stored in a dry place at room temperature (15-30℃).
Required Reagents and Equipment
- Thermomixer
Instructions
Preparations
- Read this instruction manual carefully before use.
- For long-term storage, keep Proteinase K at -20 °C.
- Check Buffer RLC for crystals or precipitates before use. If present, dissolve completely by water bath incubation at 56 °C.
- Tissue sample pretreatment: Place 20 mg tissue sample into a self-provided 1.5 mL centrifuge tube, add 500 μL Buffer RLC, and homogenize with a tissue homogenizer. Centrifuge at 12,000 rpm (~13,400×g) for 1 min, and take 200 μL of the supernatant as the sample.
Protocol
- Take a self-provided 1.5 mL centrifuge tube, add 500 μL Buffer RLC, 200 μL sample, and 20 μL Proteinase K. Vortex for 5 seconds, then incubate at room temperature with shaking at 1,200 rpm on a ThermoMixer for 10 min.
For wet swab samples, take 200 μL for extraction after thorough vortexing. For dry swab samples, soak in 400 μL normal saline, vortex thoroughly and let stand for 5 min. Centrifuge at 12,000 rpm for 1 min, then take 200 μL supernatant for extraction.
- Briefly centrifuge the tube. Transfer the solution from Step 1 into a Spin Column DM assembled in a collection tube. Centrifuge at 12,000 rpm (~13,400×g) for 1 min, discard the waste liquid, and place the spin column back into the collection tube.
- Add 500 μL Buffer PGWT to the spin column. Centrifuge at 12,000 rpm for 1 min, discard the waste liquid, and reassemble the column.
- Add 500 μL Buffer GWT2 to the spin column. Centrifuge at 12,000 rpm for 1 min, discard the waste liquid, and reassemble the column.
- Centrifuge at 12,000 rpm for 2 min and discard the waste liquid. Place the spin column at room temperature for 2 min to dry.
- Place the spin column into a new RNase-Free Centrifuge Tube. Pipette 40–100 μL RNase-Free Water vertically onto the center of the column membrane without contact. Let stand at room temperature for 2 min, then centrifuge at 12,000 rpm for 1 min to collect the nucleic acid solution. Store at -80 °C for long-term preservation.