T4 RNA ligase 2 HYJ237
T4 RNA Ligase 2, also known as T4 RNA Ligase 2, is an ATP-dependent double-stranded RNA ligase (dsRNA Ligase), capable of performing both intramolecular circular ligation and intermolecular linear ligation of double-stranded RNA. Unlike T4 RNA Ligase 1, T4 RNA Ligase 2 exhibits significantly greater ligation activity towards nicks within double-stranded RNA than towards the ends of single-stranded RNA. T4 RNA Ligase 2 can also be used to connect the 3' hydroxyl group of an RNA strand to the 5' phosphate group of a DNA strand, either intramolecularly or intermolecularly within double-stranded nucleic acids (RNA duplexes, RNA/DNA hybrids, or DNA duplexes). T4 RNA Ligase 2 requires both a 5' phosphate and a 3' hydroxyl group, enabling the ligation reaction between the 5' phosphate group of an RNA or DNA strand and the 3' hydroxyl group of an RNA strand. It is primarily used to connect nicks in double-stranded RNA (Double-stranded RNA sticky-end ligation). It can also be used to connect nicks in double-stranded structures, such as the 3' hydroxyl group of RNA and the 5' phosphate group of DNA. T4 RNA Ligase 2 is a recombinant protein expressed in Escherichia coli. The gene expressed is derived from the T4 bacteriophage.
Components
|
Components |
1KU |
5KU |
|
T4 RNA Ligase 2 (10U/µL) |
100 uL |
500 uL |
|
10× Reaction Buffer |
300 uL |
1.5 mL |
|
RNase-Free ddH2O |
1 mL |
5 mL |
Storage
Store at -25 to -15°C. Valid for 1 years.
Storage buffer
10mM Tris-HCl(pH7.5), 50mM KCl, 35mM(NH4)2SO4, 0.1mM EDTA, 0.1mM DTT , 50% (v/v) Glycerol.
Activity Definition
One unit is defined as the amount of enzyme required to ligate 0.4 µg of an equimolar mixture of 23-mer and 17-mer RNAs in 20 µL at 37°C in 30 minutes.
Quality Control
1.Free of RNase and DNase contamination.
2.T4 RNA Ligase 2 purity >90% as determined by Coomassie Brilliant Blue-stained SDS-polyacrylamide gel.