T7 RNA Polymerase ELISA kit (Static version) HYJ524
This kit uses the double antibody sandwich method: The microwell plate has been pre-coated with anti-T7 RNA polymerase antibody. The sample is added and incubated with horseradish peroxidase (HRP) labeled antibody to form antibody-antigen-enzyme-labeled antibody complex. After washing, Then TMB substrate solution is added and catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The depth of the color is positively correlated with the T7 RNA polymerase content in the sample. The absorbance (OD value) is measured at 450nm.
Components
|
Components |
48 T |
96 T |
|
ELISA microplate |
48 T |
96 T |
|
Standard concentrate (0.1mg/ml) |
0.025mL |
0.05mL |
|
Enzyme conjugate (100×) |
0.04mL |
0.08mL |
|
Sample dilution buffer |
15mL |
30mL |
|
Enzyme conjugate dilution buffer |
4mL |
8mL |
|
Wash concentrate (10×) |
15mL |
30mL |
|
Substrate solution |
6mL |
12mL |
|
Stop solution |
3mL |
6mL |
|
Sealing film |
2 films |
4 films |
Storage
Store at 2-8℃ for 12 months.
Additional Materials Required
Microplate reader with 450±10nm filter(better if can detect at 450 and 650 nm wavelength).