T7 RNA Polymerase HYJ211
T7 RNA polymerase is a DNA-dependent RNA polymerase derived from the bacteriophage T7. It possesses highly specific 5'→3' RNA polymerase activity. T7 RNA polymerase is highly specific for the T7 promoter and can synthesize large amounts of RNA using the downstream sequence of the T7 promoter as a template.
Components
|
Components |
5KU |
50KU |
500KU |
5000KU |
|
T7 RNA Polymerase (50 U/μL) |
0.1 mL |
1 mL |
10 mL |
100 mL |
|
10×HH T7 Buffer |
2 ×0.1 mL |
2 ×1 mL |
2 × 10 mL |
2 × 100 mL |
Storage
Transport below 0°C; store at -25 to -15°C. Valid for 24 months.
Storage buffer
50 mM Tris-HCl, 100 mM NaCl, 20mM β-ME, 1 mM EDTA, 50% Glycerol, 0.1% (w/v) Triton X-100, pH 7.9 @ 25°C
Activity Definition
One activity unit (U) is defined as the amount of enzyme required to catalyze the conversion of NTP to 1 nmol of PPi in a standard reaction system at 37°C within 1 hour.
Quality Control
1. Endonuclease Residue Detection: 50U of this enzyme and 1 μg of λDNA were added to a 50 μL reaction system and incubated at 37°C for 16 hours. The DNA bands on agarose gel electrophoresis remained unchanged.
2. Exonuclease Residue Detection: 50 U of this enzyme and 1 μg of λ-Hind III digested DNA were added to a 50μL reaction system and incubated at 37°C for 16 hours. The DNA bands on agarose gel electrophoresis remained unchanged.
3. NickaseResidue Detection: 50U of this enzyme and 1 μg of pBR322 DNA were added to a 50 μL reaction system and incubated at 37°C for 16 hours. The DNA bands on agarose gel electrophoresis remained unchanged.
4. RNase residual test: Add 50U of this enzyme and 1.6 μg of MS2 RNA to a 50 μL reaction system and incubate at 37°C for 16 hours. The RNA bands observed on agarose gel electrophoresis remain unchanged.
5. Heat inactivation: 75°C for 10 minutes.