T7 RNA Poymerase (High concentration)
Description
T7 RNA Polymerase is a DNA-dependent RNA-olymerase from T7 phage ， that possesses a strong and specific 5 ´→ 3 ´ RNA polymerase activity. T7 RNA Poplymerase has high specificity for T7 promoter sequences and will synthesize large quantities of RNA from a DNA fragment inserted downstream from a promoter.
Chemical Structure
Unit Definition
One unit is defined as the amount of enzyme required to incorporate 1 nmol ATP into an acid-insoluble material in 1 hour at 37°C
Specification
| Test Items | Specifications |
| Protein Purity | ≥95% |
| Nickase | None detected |
| Rnase | None detected |
| Endonuclease | None detected |
| Exonuclease | None detected |
| Promoter Specificity | Not detectable of nonspecific product |
| E.coli gDNA | ≤0.1 pg/50U |
| Endotoxin | LAL-Test, ≤10EU/mg |
Transportation and storage
Transportation: under 0 °C
Storage: All components should be stored at -25～-15°C
Recommended re-test Life: 2 year
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