Trypsin Residue Detection Kit (ELISA) HYI134
This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Porcine trypsin antibody. Trypsin present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Porcine trypsin antibody is added and binds to trypsin in the sample. After washing, HRP-Streptavidin is added and binds to the Biotinylated trypsin antibody. After incubation unbound HRP-Streptavidin is washed away. Then TMB substrate solution is added and catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of trypsin sample captured in plate. The absorbance is measured at 450 nm.
Product specifications
Recombinant Trypsin is frequently used in biopharmaceutical manufacturing-during cell preparation or for the modification and activation of products. Trypsin poses safety risks and must therefore be removed before final product release. This Sandwich Kit is for quantitative measurement of Residual Trypsin in cell culture supernatant and other procedures in biopharmaceutical manufacturing when Trypsin is used.
Components
|
Reagent |
96 T |
|
ELISA microplate |
8×12 wells |
|
Biotinylated detection antibody (100x) |
120 μL |
|
HRP-streptavidin (100x) |
120 μL |
|
Dilution buffer |
45 mL |
|
TMB substrate solution |
12 mL |
|
Stop solution |
6 mL |
|
concentrated wash buffer (20x) |
35 mL |
|
Standard (100ng/mL) |
0.5mL |
|
Sealing film |
4 films |
Transportation and storage
1. For unused kit: The whole kit could be stored at 2~8℃ in shelf life. Strong light should be avoided for storage stability.
2. For used kit: Once the microplate is opened, please cover unused wells with plate sealer and return to the foil pouch containing the desiccant pack, zip-seal the foil pouch and return to 2~8℃ as soon as possible after use. Other reagents should be returned to 2~8℃ as soon as possible after use.
Materials Required But Not Supplied
1. Microplate reader with 450±10nm filter(better if can detect at 450 and 650 nm wavelength).
2. Microplate shaker.
3. 37℃ incubator.