Ultra Nuclease Assay Kit (ELISA) Cat.No.: HYI133
This UltraNuclease ELISA kit is a sandwich ELISA to be performed in a microplate format. Sample potentially containing endonuclease are incubated in microtiter plate wells which have been pre-coated with an affinity purified anti-endonuclease capture antibody. After incubation and a washing step in which unbound components are removed, a biotin-conjugated, anti-endonuclease detector antibody is added. After further washing steps, the bound detector antibody reacts with an enzyme conjugate acting as tracer. After a final washing step, a substrate solution is added to the wells and reacted, resulting in color development. The optical density is measured photometrically and is proportional to the analyte concentration present in the wells. The endonuclease concentration in unknown samples can be calculated based on the corresponding standard curve.
Components
|
Reagent |
96 T |
|
ELISA microplate |
8×12 wells |
|
Detector antibody (100×) |
0.15mL×1 |
|
Enzyme conjugate (1000×) |
15μL×1 |
|
Dilution buffer |
50mL×1 |
|
Substrate solution |
12mL×1 |
|
Stop solution |
6mL×1 |
|
Wash concentrate (10×) |
60mL×1 |
|
Standard concentrate |
0.1mL×1 |
|
Sealing film |
4 films |
Note: Control standard endotoxin is freeze-dried powder. See label for titer and reconstitution volume.
Storage and stability
Unopened kit should be stored at 2-8℃ for stability until the expiration date printed on the kit. Opened reagents should be stored at 2-8℃ and are stable for 6 weeks.
Equipment required
1. Microplate reader spectrophotometer withdual wavelength capability at 450 and 650nm. (If your plate reader does not provide dual wavelength analysis you may read at just the 450nm wavelength.)
2. Microtiter plate rotator (400-600 rpm).