Ultra Nuclease Assay Kit(ELISA)

This Ultra Nuclease ELISA kit is a sand wich ELISA to be performed in a microplate format. Sample potentially containing endonuclease are incubated in microtiter plate wells which have been pre-coated with an affinity purified anti-endonuclease capture antibody. After incubation and a washing step in which unbound components are removed, an enzyme-conjugated, anti-endonuclease detector antibody is added. The immunological reactions result in the formation of a sandwich complex of solid phase antibody-endonuclease-enzyme labeled antibody. After a final washing step, a substrate solution is added to the wells and reacted, resulting in color development. The optical density is measured photometrically and is proportional to the
analyte concentration present in the wells. The endonuclease concentration in unknown samples can be calculated based on the corresponding standard curve.